Purification and characterization of latent polyphenol oxidase from truffles (Terfezia arenaria).

Antioxidants / chemistry Ascomycota / chemistry Catechol Oxidase / chemistry Catechols / chemistry Cysteine / chemistry Hydrogen-Ion Concentration Phenols / chemistry Pyrones / chemistry Sodium Fluoride / chemistry Substrate Specificity Sulfites / chemistry Temperature
,kojic acid.enzymatic browning Polyphenol oxidase Purification, characterization Truffles

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Feb 2020
Historique:
received: 25 08 2019
revised: 26 09 2019
accepted: 28 09 2019
pubmed: 30 11 2019
medline: 20 11 2020
entrez: 29 11 2019
Statut: ppublish

Résumé

The polyphenol oxidase was extracted and purified from truffles (Terfezia arenaria) and it exhibited a molecular weight of 67 kDa. The truffle PPO was able to oxidize monophenolic, o-diphenolic and triphenolic substrates. Thus, the enzyme seems to be stable under wide range of pH and temperature. Best catalytic efficiency was observed for catechol as substrate (Kcat/km; 674.2 S

Identifiants

DOI: 10.1016/j.ijbiomac.2019.09.126 PMID: 31778703
pubmed: 31778703
pii: S0141-8130(19)36831-X
doi: 10.1016/j.ijbiomac.2019.09.126
pii:
doi:

Substances chimiques

Antioxidants 0
Catechols 0
Phenols 0
Pyrones 0
Sulfites 0
sodium metabisulfite 4VON5FNS3C
kojic acid 6K23F1TT52
Sodium Fluoride 8ZYQ1474W7
Catechol Oxidase EC 1.10.3.1
Cysteine K848JZ4886
catechol LF3AJ089DQ

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

885-893

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Farouk Benaceur (F)

Laboratory of Biological and Agricultural Sciences (LSBA), University of Amar Telidji (UATL), 03000 Laghouat, Algeria. Electronic address: f.benaceur@lagh-univ.dz.

Rachid Chaibi (R)

Laboratory of Biological and Agricultural Sciences (LSBA), University of Amar Telidji (UATL), 03000 Laghouat, Algeria.

Fethi Berrabah (F)

Laboratory of Exploration and Valorization of Steppic Ecosystems, Faculty of Nature and Life Sciences, University of Ziane Achour, 17000 Djelfa, Algeria.

Aref Neifar (A)

Laboratory of Molecular Biotechnology of Eukaryotes, Biotechnology Center of Sfax, University of Sfax, Tunisia; Laboratory of Aqua, National Institute of Sciences and Technologies of the Sea (INSTM), Sfax, Tunisia.

Mourad Leboukh (M)

Laboratory of Biological and Agricultural Sciences (LSBA), University of Amar Telidji (UATL), 03000 Laghouat, Algeria.

Kenza Benaceur (K)

Laboratory of Biological and Agricultural Sciences (LSBA), University of Amar Telidji (UATL), 03000 Laghouat, Algeria.

Wafa Nouioua (W)

Department of English, Faculty of Letters and Languages, University of Amar Telidji (UATL), 03000 Laghouat, Algeria.

Asmaa Rezzoug (A)

Laboratory of Biological and Agricultural Sciences (LSBA), University of Amar Telidji (UATL), 03000 Laghouat, Algeria.

Houria Bouazzara (H)

Laboratory of Biological and Agricultural Sciences (LSBA), University of Amar Telidji (UATL), 03000 Laghouat, Algeria.

Hicham Gouzi (H)

Laboratory of Biological and Agricultural Sciences (LSBA), University of Amar Telidji (UATL), 03000 Laghouat, Algeria. Electronic address: h.gouzi@lagh-univ.dz.

Hubert Cabana (H)

Department of Civil Engineering, Université de Sherbrooke, 2500 Boulevard de l'Université, Sherbrooke, Québec J1K 2R1, Canada.

Ali Gargouri (A)

Laboratory of Molecular Biotechnology of Eukaryotes, Biotechnology Center of Sfax, University of Sfax, Tunisia.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Antioxydants › Purification and characterization of latent...
questionsmedicales.fr › Eucaryotes › Champignons › Ascomycota › Purification and characterization of latent...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxygénases › Mixed function oxygenases › Catechol oxidase › Purification and characterization of latent...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures cycliques › Hydrocarbures aromatiques › Dérivés du benzène › Phénols › Catéchols › Purification and characterization of latent...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés soufrés › Cystéine › Purification and characterization of latent...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Purification and characterization of latent...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures cycliques › Hydrocarbures aromatiques › Dérivés du benzène › Phénols › Purification and characterization of latent...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyrannes › Pyrones › Purification and characterization of latent...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Cariostatiques › Fluorure de sodium › Purification and characterization of latent...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Purification and characterization of latent...
questionsmedicales.fr › Produits chimiques inorganiques › Sulfites › Purification and characterization of latent...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Purification and characterization of latent...