Supplementation of l-tryptophan (an aromatic amino acid) in tris citric acid extender enhances post-thaw progressive motility, plasmalemma, mitochondrial membrane potential, acrosome, and DNA integrities, and in vivo fertility rate of buffalo (Bubalus bubalis) bull spermatozoa.
Acrosome
Animals
Bicarbonates
/ chemistry
Birth Rate
Buffaloes
Cell Membrane
Citric Acid
/ chemistry
Cryopreservation
/ methods
Cryoprotective Agents
/ chemistry
DNA
Female
Fertility
/ drug effects
Freezing
Humans
Male
Membrane Potential, Mitochondrial
/ physiology
Semen Analysis
Semen Preservation
/ methods
Sperm Motility
/ drug effects
Spermatozoa
/ drug effects
Tromethamine
/ chemistry
Tryptophan
/ pharmacology
Buffalo spermatozoa
DNA integrity
In vivo fertility
Mitochondrial membrane potential
Semen quality
l-tryptophan
Journal
Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252
Informations de publication
Date de publication:
01 02 2020
01 02 2020
Historique:
received:
27
07
2019
revised:
11
11
2019
accepted:
25
11
2019
pubmed:
30
11
2019
medline:
17
9
2020
entrez:
30
11
2019
Statut:
ppublish
Résumé
The aromatic amino acid l-tryptophan is an essential and versatile molecule, acts by transferring an electron to free radicals and protects the plasma membrane from injuries. The aim of the present study was to investigate the effects of l-tryptophan in extender on semen quality parameters, in vitro longevity and in vivo fertility rate of buffalo spermatozoa during cryopreservation. Two ejaculates were collected from each bull (n = 2 ejaculates and n = 4 bulls) with artificial vagina at 42 °C followed by initial evaluation for volume, motility, concentrations and were diluted in five extenders (C = lacking l-tryptophan, D1 = 25 μ M l-tryptophan, D2 = 50 μ M l-tryptophan, D3 = 75 μ M l-tryptophan, and D4 = 100 μ M l-tryptophan) respectively, and cryopreserved. The experiment was repeated four times (n = 4 replicates). At post-dilution, sperm plasma membrane integrity (PMI, %), supravital plasma membrane integrity (SVPMI, %), hypo-resistivity (HR, %) and acrosome integrity (ACR-I, %) were significantly higher (P < 0.05) in extender supplemented with D4 than control. At post-thawing, progressive motility (PM, %), PMI, SVPMI, HR, ACR-I, and DNA-I of buffalo bull spermatozoa were significantly higher in D4 than control. Sperm in vitro longevity (%) assessed in terms of PM, SVPMI, and ACR-1 were significantly higher in D4 than control. Sperm mitochondrial membrane potential (%) was higher in treated groups than the control. The in vivo fertility rate was significantly higher in D4 than control (60.17% vs. 44.17%, P < 0.05). It is concluded that the supplementation of l-tryptophan in tris citric acid extender improves semen quality parameters, in vitro longevity and in vivo fertility rate of buffalo spermatozoa during freezing and thawing process.
Identifiants
pubmed: 31783000
pii: S0011-2240(19)30236-6
doi: 10.1016/j.cryobiol.2019.11.044
pii:
doi:
Substances chimiques
Bicarbonates
0
Cryoprotective Agents
0
Tromethamine
023C2WHX2V
Tribonate
133249-32-8
Citric Acid
2968PHW8QP
Tryptophan
8DUH1N11BX
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
117-123Informations de copyright
Copyright © 2019. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors have no conflict of interest in the publication of this research article.