LMB-1 producing Citrobacter freundii from Argentina, a novel player in the field of MBLs.

Argentina Bacterial Proteins / biosynthesis Carbapenem-Resistant Enterobacteriaceae / metabolism Citrobacter freundii / enzymology Escherichia coli / genetics Genes, Bacterial Microbial Sensitivity Tests Whole Genome Sequencing beta-Lactamases / metabolism
CPE Carbapenemase Class B3 MBL Metallo-beta-lactamase

Journal

International journal of antimicrobial agents
ISSN: 1872-7913
Titre abrégé: Int J Antimicrob Agents
Pays: Netherlands
ID NLM: 9111860

Informations de publication

Date de publication:
Feb 2020
Historique:
received: 26 07 2019
revised: 18 11 2019
accepted: 24 11 2019
pubmed: 1 12 2019
medline: 24 11 2020
entrez: 1 12 2019
Statut: ppublish

Résumé

Carbapenemase-producing Enterobacterales expressing OXA-48, KPC, NDM, VIM or IMP enzymes are increasingly reported worldwide. We have characterized LMB-1, a novel metallo-β-lactamase (MBL) of Ambler class B3 from Citrobacter freundii 164 (Cf164) clinical isolate from Buenos Aires, Argentina. Cf164 displayed reduced susceptibility to carbapenems but gave inconsistent results with carbapenemase confirmatory tests, indicating the presence of a weak carbapenemase. Analysis of whole-genome sequencing (WGS) of Cf164 using Resfinder revealed four β-lactamase genes coding for CTX-M-8, PER-2, TEM-1 and CMY-150, a novel chromosomally-encoded CMY variant. Kinetic parameters of purified CMY-150 did not reveal any carbapenemase activity. However, CMY-150 conferred higher minimum inhibitory concentrations (MICs) to E. coli for ceftazidime and aztreonam compared with CMY-2. The in-house-developed β-lactamase search software (ResMiner) in WGS data revealed a novel subclass B3 MBL named LMB-1. LMB-1 conferred resistance to penicillins and expanded-spectrum cephalosporins and reduced susceptibility to carbapenems in E. coli. The bla

Identifiants

DOI: 10.1016/j.ijantimicag.2019.11.014 PMID: 31785341
pubmed: 31785341
pii: S0924-8579(19)30328-0
doi: 10.1016/j.ijantimicag.2019.11.014
pii:
doi:

Substances chimiques

Bacterial Proteins 0
beta-Lactamases EC 3.5.2.6
carbapenemase EC 3.5.2.6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

105857

Informations de copyright

Copyright © 2019 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

Auteurs

Laura Dabos (L)

EA7361 "Structure, dynamic, function and expression of broad spectrum β-lactamases", Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France; Joint research Unit EERA « Evolution and Ecology of Resistance to Antibiotics », Institut Pasteur-APHP-University Paris Sud, Paris, France.

Carlos H Rodriguez (CH)

Departamento de Bioquímica Clinica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

Marcela Nastro (M)

Departamento de Bioquímica Clinica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

Laurent Dortet (L)

EA7361 "Structure, dynamic, function and expression of broad spectrum β-lactamases", Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France; Joint research Unit EERA « Evolution and Ecology of Resistance to Antibiotics », Institut Pasteur-APHP-University Paris Sud, Paris, France; Department of Bacteriology-Hygiene, Bicêtre Hospital, APHP, Le Kremlin-Bicêtre, France; French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France.

Rémy A Bonnin (RA)

EA7361 "Structure, dynamic, function and expression of broad spectrum β-lactamases", Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France; Joint research Unit EERA « Evolution and Ecology of Resistance to Antibiotics », Institut Pasteur-APHP-University Paris Sud, Paris, France; French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France.

Angela Famiglietti (A)

Departamento de Bioquímica Clinica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

Bogdan I Iorga (BI)

Université Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, UPR 2301, Labex LERMIT, 91198, Gif-sur-Yvette, France.

Carlos Vay (C)

Departamento de Bioquímica Clinica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

Thierry Naas (T)

EA7361 "Structure, dynamic, function and expression of broad spectrum β-lactamases", Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France; Joint research Unit EERA « Evolution and Ecology of Resistance to Antibiotics », Institut Pasteur-APHP-University Paris Sud, Paris, France; Department of Bacteriology-Hygiene, Bicêtre Hospital, APHP, Le Kremlin-Bicêtre, France; French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France. Electronic address: thierry.naas@aphp.fr.

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Classifications MeSH

questionsmedicales.fr › Régions géographiques › Amériques › Amérique du Sud › Argentine › LMB-1 producing Citrobacter freundii from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › LMB-1 producing Citrobacter freundii from...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Enterobacteriaceae résistantes aux carbapénèmes › LMB-1 producing Citrobacter freundii from...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Citrobacter › Citrobacter freundii › LMB-1 producing Citrobacter freundii from...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › LMB-1 producing Citrobacter freundii from...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Gènes bactériens › LMB-1 producing Citrobacter freundii from...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Tests de sensibilité microbienne › LMB-1 producing Citrobacter freundii from...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Séquençage du génome entier › LMB-1 producing Citrobacter freundii from...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Amidohydrolases › bêta-Lactamases › LMB-1 producing Citrobacter freundii from...