Efficient expression of chondroitinase ABC I for specific disaccharides detection of chondroitin sulfate.

Chemical Phenomena Chondroitin ABC Lyase / chemistry Chondroitin Sulfates / analysis Chromatography, High Pressure Liquid Disaccharides / analysis Fermentation Hydrogen-Ion Concentration Recombinant Proteins / genetics Temperature
Chondroitin sulfate Chondroitinase ABC I Signal peptide Soluble expression

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Jan 2020
Historique:
received: 06 10 2019
revised: 25 11 2019
accepted: 26 11 2019
pubmed: 2 12 2019
medline: 6 10 2020
entrez: 2 12 2019
Statut: ppublish

Résumé

Chondroitinase ABC I (ChSase ABC I) is a key enzyme of chondroitin sulfate (CS) degradation and widely used for CS detection in the medicine filed. However, the recombinant ChSase ABC I was weakly expressed in Escherichia coli because the forms of it were mostly inclusion bodies. In this study, a signal peptide (pelB) was used for the soluble form expression of ChSase ABC I in E. coli. Then the culture condition for ChSase ABC I expression was optimized through response surface methodology. Results revealed that the expression level of ChSase ABC I in a 7.5 L fermentor (29.03 mL

Identifiants

DOI: 10.1016/j.ijbiomac.2019.11.215 PMID: 31786296
pubmed: 31786296
pii: S0141-8130(19)38064-X
doi: 10.1016/j.ijbiomac.2019.11.215
pii:
doi:

Substances chimiques

Disaccharides 0
Recombinant Proteins 0
Chondroitin Sulfates 9007-28-7
Chondroitin ABC Lyase EC 4.2.2.20

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

41-48

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Xingyu Lu (X)

College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, China.

Qian Zhong (Q)

College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, China.

Jian Liu (J)

College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, China.

Fulin Yang (F)

College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, China.

Chenghui Lu (C)

College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, China.

Huan Xiong (H)

College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, China.

Sha Li (S)

College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, China; College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China.

Yibo Zhu (Y)

College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, China.

Lingtian Wu (L)

College of Biological and Food Engineering, Changshu Institute of Technology, 99 South Third Ring Road, Changshu 215500, China; College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China. Electronic address: wlt913@cslg.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Efficient expression of chondroitinase ABC I...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-oxygen lyases › Polysaccharide-lyases › Chondroitinases et chondroitin lyases › Chondroitine lyases › Chondroitine ABC lyase › Efficient expression of chondroitinase ABC I...
questionsmedicales.fr › Glucides › Polyosides › Glycosaminoglycanes › Chondroïtine › Chondroïtines sulfate › Efficient expression of chondroitinase ABC I...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Efficient expression of chondroitinase ABC I...
questionsmedicales.fr › Glucides › Sucres › Diholoside › Efficient expression of chondroitinase ABC I...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › Efficient expression of chondroitinase ABC I...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Efficient expression of chondroitinase ABC I...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Efficient expression of chondroitinase ABC I...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Efficient expression of chondroitinase ABC I...