Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6.

Bacterial Proteins / genetics Gene Expression Regulation, Bacterial Lignin / metabolism Metabolic Networks and Pathways / genetics Oxidoreductases, O-Demethylating / genetics Promoter Regions, Genetic / genetics Repressor Proteins / metabolism Sphingomonadaceae / physiology Transcription, Genetic Vanillic Acid / metabolism

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
02 12 2019
Historique:
received: 29 07 2019
accepted: 12 11 2019
entrez: 4 12 2019
pubmed: 4 12 2019
medline: 18 11 2020
Statut: epublish

Résumé

Vanillate and syringate are major intermediate metabolites generated during the microbial degradation of lignin. In Sphingobium sp. SYK-6, vanillate is O demethylated to protocatechuate by LigM; protocatechuate is then catabolized via the protocatechuate 4,5-cleavage pathway. Syringate is O demethylated to gallate by consecutive reactions catalyzed by DesA and LigM, and then gallate is subjected to ring cleavage by DesB. Here, we investigated the transcriptional regulation of desA, ligM, and desB involved in vanillate and syringate catabolism. Quantitative reverse transcription-PCR analyses indicated that the transcription of these genes was induced 5.8-37-fold in the presence of vanillate and syringate. A MarR-type transcriptional regulator, SLG_12870 (desR), was identified as the gene whose product bound to the desB promoter region. Analysis of a desR mutant indicated that the transcription of desB, ligM, and desR is negatively regulated by DesR. Purified DesR bound to the upstream regions of desB, ligM, and desR, and the inverted repeat sequences similar to each other in these regions were suggested to be essential for DNA binding of DesR. Vanillate and syringate inhibited DNA binding of DesR, indicating that these compounds are effector molecules of DesR. The transcription of desA was found to be regulated by an as-yet unidentified regulator.

Identifiants

DOI: 10.1038/s41598-019-54490-7 PMID: 31792252 PMC: PMC6888825
pubmed: 31792252
doi: 10.1038/s41598-019-54490-7
pii: 10.1038/s41598-019-54490-7
pmc: PMC6888825
doi:

Substances chimiques

Bacterial Proteins 0
Repressor Proteins 0
Lignin 9005-53-2
Oxidoreductases, O-Demethylating EC 1.-
vanillate O-demethylase EC 1.14.13.82
Vanillic Acid GM8Q3JM2Y8

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

18036

Références

Plant Physiol. 2010 Jul;153(3):895-905
pubmed: 20472751
Int Microbiol. 2005 Sep;8(3):195-204
pubmed: 16200498
PLoS One. 2014 Mar 21;9(3):e92249
pubmed: 24657997
J Mol Cell Biol. 2010 Oct;2(5):243-54
pubmed: 20716550
J Bacteriol. 2004 Aug;186(15):4951-9
pubmed: 15262932
FEMS Microbiol Lett. 2007 Sep;274(2):323-8
pubmed: 17645527
Biochem J. 2000 Sep 15;350 Pt 3:609-29
pubmed: 10970772
Appl Environ Microbiol. 2009 Aug;75(16):5195-201
pubmed: 19542348
J Bacteriol. 2010 Jul;192(13):3394-405
pubmed: 20435721
Environ Microbiol. 2002 Dec;4(12):824-41
pubmed: 12534466
Annu Rev Plant Biol. 2003;54:519-46
pubmed: 14503002
Biochemistry. 2018 May 15;57(19):2837-2845
pubmed: 29658701
J Biotechnol. 2016 Oct 20;236:110-9
pubmed: 27544286
J Bacteriol. 2004 May;186(9):2757-65
pubmed: 15090517
Nat Prod Rep. 2011 Nov;28(12):1883-96
pubmed: 21918777
J Bacteriol. 1999 Jun;181(11):3494-504
pubmed: 10348863
Curr Microbiol. 2005 Jul;51(1):59-65
pubmed: 15971090
FEMS Microbiol Lett. 2000 Jun 1;187(1):65-8
pubmed: 10828402
Nucleic Acids Res. 2003 Nov 15;31(22):6598-609
pubmed: 14602920
Biotechnol Biofuels. 2019 Feb 15;12:32
pubmed: 30815030
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
New Phytol. 2010 Jul;187(2):273-85
pubmed: 20642725
Appl Microbiol Biotechnol. 2003 Jun;61(5-6):528-35
pubmed: 12764569
Biotechnol Adv. 2019 Nov 1;37(6):107360
pubmed: 30959173
Appl Environ Microbiol. 2018 Oct 30;84(22):
pubmed: 30217841
Biosci Biotechnol Biochem. 2007 Jan;71(1):1-15
pubmed: 17213657
J Gen Appl Microbiol. 2006 Apr;52(2):131-5
pubmed: 16778358
J Bacteriol. 2015 Mar;197(5):959-72
pubmed: 25535273
FEMS Microbiol Lett. 2012 Jul;332(1):68-75
pubmed: 22515452
Appl Environ Microbiol. 2018 Oct 1;84(20):
pubmed: 30120118
BMC Microbiol. 2018 Oct 24;18(1):154
pubmed: 30355315
Curr Opin Biotechnol. 2016 Dec;42:40-53
pubmed: 26974563
FEBS J. 2015 Apr;282(7):1190-213
pubmed: 25649492
J Bacteriol. 2005 Aug;187(15):5067-74
pubmed: 16030198
Curr Biol. 2009 Jan 27;19(2):169-75
pubmed: 19167225
J Bacteriol. 2005 Mar;187(6):2030-7
pubmed: 15743951
Environ Microbiol Rep. 2017 Dec;9(6):679-705
pubmed: 29052962
Nucleic Acids Res. 2007;35(20):e137
pubmed: 17959646
Crit Rev Biochem Mol Biol. 2017 Dec;52(6):595-613
pubmed: 28670937
Microb Biotechnol. 2009 Mar;2(2):164-77
pubmed: 21261911
Appl Environ Microbiol. 2012 Jan;78(2):586-8
pubmed: 22057861
Bioresour Technol. 2019 Aug;285:121327
pubmed: 30991184
J Bacteriol. 1999 Jan;181(1):55-62
pubmed: 9864312
Appl Environ Microbiol. 2017 Mar 31;83(8):
pubmed: 28188206
Chem Rev. 2010 Jun 9;110(6):3552-99
pubmed: 20218547

Auteurs

Takuma Araki (T)

Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.
Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687, Japan.

Shusuke Umeda (S)

Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.

Naofumi Kamimura (N)

Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.

Daisuke Kasai (D)

Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.

Shuta Kumano (S)

Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.

Tomokuni Abe (T)

Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.

Chika Kawazu (C)

Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.

Yuichiro Otsuka (Y)

Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687, Japan.

Masaya Nakamura (M)

Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687, Japan.

Yoshihiro Katayama (Y)

College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan.

Masao Fukuda (M)

Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.
Department of Biological Chemistry, Chubu University, Kasugai, Aichi, 487-8501, Japan.

Eiji Masai (E)

Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan. emasai@vos.nagaokaut.ac.jp.

Articles similaires

Atomic view of photosynthetic metabolite permeability pathways and confinement in synthetic carboxysome shells.

Daipayan Sarkar, Christopher Maffeo, Markus Sutter et al.
1.00
Photosynthesis Ribulose-Bisphosphate Carboxylase Carbon Dioxide Molecular Dynamics Simulation Cyanobacteria

Menthol as an effective inhibitor of quorum sensing and biofilm formation in Candida albicans and Candida glabrata by targeting the transcriptional repressor TUP1.

Pouria Khodavandi, Maryam Miri Soogh, Fahimeh Alizadeh et al.
1.00
Biofilms Candida albicans Quorum Sensing Candida glabrata Menthol

A computational model for bacteriophage ϕX174 gene expression.

Alexis M Hill, Tanvi A Ingle, Claus O Wilke
1.00
Gene Expression Regulation, Viral Promoter Regions, Genetic Bacteriophage phi X 174 Computer Simulation Models, Genetic

Two codependent routes lead to high-level MRSA.

Abimbola Feyisara Adedeji-Olulana, Katarzyna Wacnik, Lucia Lafage et al.
1.00
Methicillin-Resistant Staphylococcus aureus Penicillin-Binding Proteins Peptidoglycan Bacterial Proteins Anti-Bacterial Agents

Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Regulation of vanillate and syringate...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Regulation of vanillate and syringate...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Biopolymères › Lignine › Regulation of vanillate and syringate...
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Regulation of vanillate and syringate...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxidoreductases, (O-demethylating) › Regulation of vanillate and syringate...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Régions promotrices (génétique) › Regulation of vanillate and syringate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéines de répression › Regulation of vanillate and syringate...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Sphingomonadaceae › Regulation of vanillate and syringate...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › Regulation of vanillate and syringate...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures cycliques › Hydrocarbures aromatiques › Dérivés du benzène › Phénols › Éthers phényliques › Éthers d'hydroxybenzoate › Acide vanillique › Regulation of vanillate and syringate...