Loss-of-Function Mutations in the ALPL Gene Presenting with Adult Onset Osteoporosis and Low Serum Concentrations of Total Alkaline Phosphatase.


Journal

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
ISSN: 1523-4681
Titre abrégé: J Bone Miner Res
Pays: United States
ID NLM: 8610640

Informations de publication

Date de publication:
04 2020
Historique:
received: 23 07 2019
revised: 11 11 2019
accepted: 23 11 2019
pubmed: 4 12 2019
medline: 29 7 2021
entrez: 4 12 2019
Statut: ppublish

Résumé

Hypophosphatasia (HPP) is a rare inherited disorder characterized by rickets and low circulating concentrations of total alkaline phosphatase (ALP) caused by mutations in ALPL. Severe HPP presents in childhood but milder forms can present in adulthood. The prevalence and clinical features of adult HPP are poorly defined. The aim of this study was to evaluate the prevalence and clinical significance of low serum total alkaline phosphatase (ALP) levels in a clinic-based population of adult osteoporotic patients. We searched for patients with low ALP in a cohort of 3285 patients referred to an osteoporosis clinic over a 10-year period and performed mutation screening of ALPL in those with low ALP (≤40 U/L) on two or more occasions. These individuals were matched with four clinic controls with a normal ALP. We also evaluated the prevalence of low ALP and ALPL mutations in 639 individuals from the general population from the same region. We identified 16/3285 (0.49%) clinic patients with low ALP and 14 (87.5%) had potentially pathogenic variants in ALPL. Eight of these individuals were heterozygous for mutations previously described in HPP and 2 were heterozygous for novel mutations (p.Arg301Trp and p.Tyr101X). These mutations were not found in clinic controls or in the general population. Eight patients with low ALP, including 4 with ALPL mutations, were treated with bisphosphonates for an average of 6.5 years. In these individuals, the rate of fractures during treatment was comparable to that in normal ALP clinic controls who were treated with bisphosphonates. We conclude that heterozygous loss-of-function mutations in ALPL are common in osteoporosis patients with low ALP. Further studies are required to determine how best these individuals should be treated. © 2019 The Authors. Journal of Bone and Mineral Research published by American Society for Bone and Mineral Research.

Identifiants

pubmed: 31793067
doi: 10.1002/jbmr.3928
pmc: PMC9328664
doi:

Substances chimiques

ALPL protein, human EC 3.1.3.1
Alkaline Phosphatase EC 3.1.3.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

657-661

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/F019394/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0700704
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K026992/1
Pays : United Kingdom

Informations de copyright

© 2019 The Authors. Journal of Bone and Mineral Research published by American Society for Bone and Mineral Research.

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Auteurs

Nerea Alonso (N)

Rheumatology and Bone Disease Unit, Centre for Genomic and Experimental Medicine, MRC institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK.

Beatriz Larraz-Prieto (B)

Rheumatology and Bone Disease Unit, Centre for Genomic and Experimental Medicine, MRC institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK.

Kathryn Berg (K)

Rheumatology and Bone Disease Unit, Centre for Genomic and Experimental Medicine, MRC institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK.

Zoe Lambert (Z)

Rheumatology and Bone Disease Unit, Centre for Genomic and Experimental Medicine, MRC institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK.

Paul Redmond (P)

Department of Psychology, University of Edinburgh, Edinburgh, UK.

Sarah E Harris (SE)

Department of Psychology, University of Edinburgh, Edinburgh, UK.
Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Edinburgh, UK.

Ian J Deary (IJ)

Department of Psychology, University of Edinburgh, Edinburgh, UK.
Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Edinburgh, UK.

Carys Pugh (C)

Division of Psychiatry, University of Edinburgh, Royal Edinburgh Hospital, Edinburgh, UK.

James Prendergast (J)

Genetics and Genomics Division, The Roslin Institute, University of Edinburgh, Midlothian, UK.

Stuart H Ralston (SH)

Rheumatology and Bone Disease Unit, Centre for Genomic and Experimental Medicine, MRC institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK.

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Classifications MeSH