Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana Using TAL Effector Nucleases.

Arabidopsis / genetics Gene Editing / methods Germ Cells / metabolism Mutagenesis, Site-Directed / methods Plants, Genetically Modified / genetics Promoter Regions, Genetic Transcription Activator-Like Effector Nucleases / genetics
Arabidopsis thaliana Genome editing Germline Heritable Knockout NHEJ Stem cell-specific promoter TALEN

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 5 12 2019
pubmed: 5 12 2019
medline: 3 2 2021
Statut: ppublish

Résumé

TAL effector nucleases (TALENs) are powerful tools to create specific knockout mutants in plants. The use of an optimized TALEN backbone and the choice of promoters that are strongly active in the stem cells of the shoot apical meristem are key to a high rate of heritable targeted mutations. Recommendations for construct design and screening for mutants are given in this chapter.

Identifiants

DOI: 10.1007/978-1-0716-0183-9_3 PMID: 31797287
pubmed: 31797287
doi: 10.1007/978-1-0716-0183-9_3
doi:

Substances chimiques

Transcription Activator-Like Effector Nucleases EC 3.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

23-30

Auteurs

Joachim Forner (J)

Organelle Biology, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam, Germany. forner@mpimp-golm.mpg.de.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Germline-Transmitted Genome Editing...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Germline-Transmitted Genome Editing...
questionsmedicales.fr › Cellules › Cellules germinales › Germline-Transmitted Genome Editing...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Mutagenèse dirigée › Germline-Transmitted Genome Editing...
questionsmedicales.fr › Stades d'organismes › Organismes génétiquement modifiés › Végétaux génétiquement modifiés › Germline-Transmitted Genome Editing...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Régions promotrices (génétique) › Germline-Transmitted Genome Editing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Protéines de fusion recombinantes › Nucléases effectrices de type activateur de transcription › Germline-Transmitted Genome Editing...