Comprehensive Analysis of the Alternaria Mycobolome Using Mass Spectrometry Based Metabolomics.
Alternaria alternata
Fourier transform ion cyclotron resonance mass spectrometry
alterperylenepoxide A-9-mercaptolactate
non-targeted metabolomics
ultra-high resolution
Journal
Molecular nutrition & food research
ISSN: 1613-4133
Titre abrégé: Mol Nutr Food Res
Pays: Germany
ID NLM: 101231818
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
22
05
2019
revised:
05
09
2019
pubmed:
6
12
2019
medline:
15
12
2020
entrez:
6
12
2019
Statut:
ppublish
Résumé
Alternaria fungi are widely distributed plant pathogens infecting grains and vegetables and causing major harvest losses in the field and during postharvest storage. Besides, consumers are endangered by the formation of toxic secondary metabolites. Some of these secondary metabolites are chemically characterized as mycotoxins, but the majority of the Alternaria mycobolome still remains unknown. Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) and LC-MS/MS are combined for the non-targeted and targeted analysis of the metabolome of three A. alternata isolates and one A. solani isolate. Due to the ultra-high resolution of FTICR-MS, unique molecular formulae are assigned to measured m/z signals. The molecular formulae are matched to entries of the databases Antibase and Kyoto Encyclopedia of Genes and Genomes. The non-targeted analysis of the fungal extracts reveals variations in the secondary metabolite profile of A. alternata and A. solani. Differences in the biosynthesis of dibenzo-α-pyrones, perylene quinones, tentoxin, and tenuazonic acid of the A. alternata and A. solani isolates are determined applying targeted LC-MS/MS. FTICR-MS analyses reveal clear differences in the metabolic profile of the A. solani and the A. alternata isolates.
Identifiants
pubmed: 31802615
doi: 10.1002/mnfr.201900558
doi:
Substances chimiques
Mycotoxins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1900558Informations de copyright
© 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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