Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications.
cytochrome P450
directed evolution
electron source engineering
enzyme mechanism
ferredoxin
light activation
metabolic engineering
protein engineering
redox partner proteins
substrate engineering
substrate specificity
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
17 01 2020
17 01 2020
Historique:
pubmed:
8
12
2019
medline:
4
9
2020
entrez:
8
12
2019
Statut:
ppublish
Résumé
Cytochrome P450 enzymes (P450s) are broadly distributed among living organisms and play crucial roles in natural product biosynthesis, degradation of xenobiotics, steroid biosynthesis, and drug metabolism. P450s are considered as the most versatile biocatalysts in nature because of the vast variety of substrate structures and the types of reactions they catalyze. In particular, P450s can catalyze regio- and stereoselective oxidations of nonactivated C-H bonds in complex organic molecules under mild conditions, making P450s useful biocatalysts in the production of commodity pharmaceuticals, fine or bulk chemicals, bioremediation agents, flavors, and fragrances. Major efforts have been made in engineering improved P450 systems that overcome the inherent limitations of the native enzymes. In this review, we focus on recent progress of different strategies, including protein engineering, redox-partner engineering, substrate engineering, electron source engineering, and P450-mediated metabolic engineering, in efforts to more efficiently produce pharmaceuticals and other chemicals. We also discuss future opportunities for engineering and applications of the P450 systems.
Identifiants
pubmed: 31811088
pii: S0021-9258(17)49939-X
doi: 10.1074/jbc.REV119.008758
pmc: PMC6970918
doi:
Substances chimiques
Steroids
0
Xenobiotics
0
Cytochrome P-450 Enzyme System
9035-51-2
Banques de données
PDB
['1OXA', '2JJN', '6B11', '1JPZ', '2X7Y', '3A4G', '2ZBX', '2ZBZ', '4OQR', '2C6H', '2C7X', '5UHU', '1OG2', '6DA2', '3N9Y', '3RUK', '4YT3', '1DT6']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
833-849Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM118122
Pays : United States
Informations de copyright
© 2020 Li et al.
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