Dynamic in vivo quantification of rod photoreceptor degeneration using fluorescent reporter mouse models of retinitis pigmentosa.
Animals
Dependovirus
Disease Models, Animal
Gene Expression
/ physiology
Genes, Reporter
/ genetics
Genetic Vectors
Green Fluorescent Proteins
/ genetics
Injections, Intraocular
Mice
Mice, Knockout
Mice, Transgenic
Ophthalmoscopy
Parvovirinae
/ genetics
Retinal Degeneration
/ metabolism
Retinal Rod Photoreceptor Cells
/ metabolism
Retinitis Pigmentosa
/ metabolism
Rhodopsin
/ genetics
Staining and Labeling
Tomography, Optical Coherence
Imaging
Retina
Retinitis pigmentosa
Scanning laser ophthalmoscopy
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
02
10
2019
revised:
25
11
2019
accepted:
04
12
2019
pubmed:
10
12
2019
medline:
18
7
2020
entrez:
10
12
2019
Statut:
ppublish
Résumé
Imaging techniques have revolutionised the assessment of retinal disease in humans and animal models. Here we describe a novel technique for the in vivo visualisation of rod photoreceptors which permits semiquantitative assessment of outer retinal degeneration, and validate this approach in two mouse models of retinitis pigmentosa (RP). Transgenic mice carrying an Nrl-EGFP allele and homozygous for either knock-out of rhodopsin (Nrl-EGFP, Rho
Identifiants
pubmed: 31816293
pii: S0014-4835(19)30737-7
doi: 10.1016/j.exer.2019.107895
pii:
doi:
Substances chimiques
enhanced green fluorescent protein
0
Green Fluorescent Proteins
147336-22-9
Rhodopsin
9009-81-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107895Subventions
Organisme : Medical Research Council
ID : MR/N00101X/1
Pays : United Kingdom
Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.