Elevated Levels of Interleukin-27 in Early Life Compromise Protective Immunity in a Mouse Model of Gram-Negative Neonatal Sepsis.


Journal

Infection and immunity
ISSN: 1098-5522
Titre abrégé: Infect Immun
Pays: United States
ID NLM: 0246127

Informations de publication

Date de publication:
20 02 2020
Historique:
received: 29 10 2019
accepted: 22 11 2019
pubmed: 11 12 2019
medline: 24 4 2020
entrez: 11 12 2019
Statut: epublish

Résumé

Neonates are at increased risk for bacterial sepsis. We established that the immune-suppressive cytokine interleukin-27 (IL-27) is elevated in neonatal mice. Similarly, human cord blood-derived macrophages express IL-27 genes and secrete more cytokine than macrophages from adults. In the present work, we hypothesized that increased levels of IL-27 predispose neonatal mice to more severe infection during Gram-negative sepsis. Serum IL-27 levels continued to rise during infection. Peripheral tissue analysis revealed systemic IL-27 expression, while myeloid cell profiling identified Gr-1- and F4/80-expressing cells as the most abundant producers of IL-27 during infection. Increased IL-27 levels were consistent with increased mortality that was improved in IL-27 receptor α (IL-27Rα)

Identifiants

pubmed: 31818960
pii: IAI.00828-19
doi: 10.1128/IAI.00828-19
pmc: PMC7035946
pii:
doi:

Substances chimiques

Interleukin-27 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2020 Seman et al.

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Auteurs

Brittany G Seman (BG)

Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA.

Jordan K Vance (JK)

Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA.

Travis W Rawson (TW)

Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA.

Michelle R Witt (MR)

Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA.

Annalisa B Huckaby (AB)

Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA.

Jessica M Povroznik (JM)

Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA.
Vaccine Development Center, West Virginia University Health Sciences Center, Morgantown, West Virginia, USA.

Shelby D Bradford (SD)

Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA.
Vaccine Development Center, West Virginia University Health Sciences Center, Morgantown, West Virginia, USA.

Mariette Barbier (M)

Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA.
Vaccine Development Center, West Virginia University Health Sciences Center, Morgantown, West Virginia, USA.

Cory M Robinson (CM)

Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA cory.robinson1@hsc.wvu.edu.
Vaccine Development Center, West Virginia University Health Sciences Center, Morgantown, West Virginia, USA.

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