Characterization of a Novel Bispecific Antibody That Activates T Cells
Animals
Antibodies, Bispecific
/ immunology
CD3 Complex
/ genetics
CD8-Positive T-Lymphocytes
/ immunology
Cell Proliferation
/ genetics
Epithelial Cell Adhesion Molecule
/ genetics
Humans
Immunoglobulin Fc Fragments
/ immunology
Mice
Neoplasms
/ immunology
Signal Transduction
/ immunology
T-Lymphocytes
/ immunology
CD3 redirection
bispecific antibodies
mechanism of action
Journal
Monoclonal antibodies in immunodiagnosis and immunotherapy
ISSN: 2167-9436
Titre abrégé: Monoclon Antib Immunodiagn Immunother
Pays: United States
ID NLM: 101590955
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
entrez:
12
12
2019
pubmed:
12
12
2019
medline:
16
5
2020
Statut:
ppublish
Résumé
Although CD3 T cell redirecting antibodies have been successfully utilized for the treatment of hematological malignancies (blinatumomab), the T cell signaling pathways induced by these molecules are incompletely understood. To gain insight into the mechanism of action for T cell redirection antibodies, we created a novel murine CD3xEpCAM bispecific antibody that incorporates a silent Fc to dissect function and signaling of murine CD8 OT1 T cells upon stimulation. T cell-mediated cytotoxicity, cytokine secretion, expression of activation markers, and proliferation were directly induced in T cells treated with the novel CD3xEpCAM bispecific molecule
Identifiants
pubmed: 31825302
doi: 10.1089/mab.2019.0035
pmc: PMC6918852
doi:
Substances chimiques
Antibodies, Bispecific
0
CD3 Complex
0
Epithelial Cell Adhesion Molecule
0
Immunoglobulin Fc Fragments
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
242-254Références
Blood Cancer J. 2015 Aug 21;5:e340
pubmed: 26295610
Immunology. 2009 Feb;126(2):165-76
pubmed: 19125887
J Leukoc Biol. 1996 Aug;60(2):278-84
pubmed: 8773590
Cell. 1994 Jan 14;76(1):17-27
pubmed: 8287475
Nat Protoc. 2014 Oct;9(10):2450-63
pubmed: 25255089
Nature. 2017 Jul 13;547(7662):217-221
pubmed: 28678778
BioDrugs. 2014 Aug;28(4):331-43
pubmed: 24638872
JCI Insight. 2018 May 17;3(10):
pubmed: 29769444
Mol Cancer Ther. 2018 Apr;17(4):776-785
pubmed: 29339550
J Med Virol. 2016 Jan;88(1):13-20
pubmed: 26104380
J Immunol. 2014 Mar 1;192(5):2143-55
pubmed: 24501199
Cancer Cell. 2017 Mar 13;31(3):383-395
pubmed: 28262555
J Immunol. 2015 May 1;194(9):4073-80
pubmed: 25888703
Nature. 1985 Apr 18-24;314(6012):628-31
pubmed: 2859527
Exp Hematol Oncol. 2017 May 2;6:12
pubmed: 28469973
Biosci Rep. 2000 Aug;20(4):213-37
pubmed: 11092246
Curr Opin Mol Ther. 2008 Jun;10(3):273-84
pubmed: 18535935
Am J Pathol. 1979 Dec;97(3):587-600
pubmed: 507192
Nature. 1985 Jul 25-31;316(6026):354-6
pubmed: 3160953
J Immunol. 1987 Aug 15;139(4):1369-74
pubmed: 3112233
Antibodies (Basel). 2017 Sep 01;6(3):null
pubmed: 31548527
Cancer Immunol Immunother. 2006 Jul;55(7):785-96
pubmed: 16187083
Pharmacol Ther. 2018 Feb;182:161-175
pubmed: 28834699
Mol Cancer Ther. 2019 Feb;18(2):312-322
pubmed: 30381448
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5145-50
pubmed: 23479652
Sci Transl Med. 2017 Oct 4;9(410):null
pubmed: 28978751
Blood. 2015 Dec 10;126(24):2578-84
pubmed: 26480933
Cancer Res. 2015 Jul 1;75(13):2587-93
pubmed: 26069246
Haematologica. 2013 Dec;98(12):1930-8
pubmed: 23812940
Int Immunol. 1999 Jul;11(7):1085-92
pubmed: 10383941
Breast Cancer Res Treat. 2017 Nov;166(2):641-650
pubmed: 28798985
J Mol Recognit. 2012 Mar;25(3):136-46
pubmed: 22407977
Mol Immunol. 2006 Feb;43(6):763-71
pubmed: 16360021
Int Immunol. 2018 Feb 3;30(1):13-22
pubmed: 29294043
Clin Immunol. 2008 Apr;127(1):107-18
pubmed: 18222733
MAbs. 2014;6(6):1571-84
pubmed: 25484061
Biomedicines. 2015 Dec 02;3(4):282-303
pubmed: 28536413
J Immunol. 2017 May 15;198(10):3939-3948
pubmed: 28424242
J Immunol. 2004 May 1;172(9):5159-67
pubmed: 15100252
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8
pubmed: 2950524
J Invest Dermatol. 2014 Jul;134(7):1884-1892
pubmed: 24468748
Sci Rep. 2017 May 30;7(1):2476
pubmed: 28559564
Nat Rev Drug Discov. 2019 Aug;18(8):585-608
pubmed: 31175342