Identification of Glyceryl MonoRicinoleate (GMR) isomers using RP-HPLC and regio-specificity of lipases.

Castor Oil / chemistry Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Glycerides / analysis Hydrolysis Isomerism Lipase / chemistry Ricinoleic Acids / analysis Ricinus / chemistry
Glyceryl MonoRicinoleate (GMR) RP-HPLC mass spectroscopy regio-specific lipase

Journal

Preparative biochemistry & biotechnology
ISSN: 1532-2297
Titre abrégé: Prep Biochem Biotechnol
Pays: England
ID NLM: 9607037

Informations de publication

Date de publication:
2020
Historique:
pubmed: 13 12 2019
medline: 25 11 2020
entrez: 13 12 2019
Statut: ppublish

Résumé

In the present study, we report a reverse-phase high-performance liquid chromatography (RP-HPLC) method for separation of the regio-isomers of Glyceryl MonoRicinoleate (GMR) identified using position specificity of lipases. The approaches explored to identify these regio-isomers include LC-mass spectrometry, UV spectroscopy, and selective hydrolysis with lipases. A distinct UV absorption spectrum and

Identifiants

DOI: 10.1080/10826068.2019.1700519 PMID: 31829798
pubmed: 31829798
doi: 10.1080/10826068.2019.1700519
doi:

Substances chimiques

Glycerides 0
Ricinoleic Acids 0
Castor Oil 8001-79-4
Lipase EC 3.1.1.3

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

401-407

Auteurs

Rajeshkumar Natwarlal Vadgama (R)

DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India.

Annamma Anil (A)

DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India.

Arvind Lali (A)

DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India.

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Classifications MeSH

questionsmedicales.fr › Mélanges complexes › Produits biologiques › Préparations à base de plantes › Huiles végétales › Huile de ricin › Identification of Glyceryl MonoRicinoleate...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Identification of Glyceryl MonoRicinoleate...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase inverse › Identification of Glyceryl MonoRicinoleate...
questionsmedicales.fr › Lipides › Glycérides › Identification of Glyceryl MonoRicinoleate...
questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › Identification of Glyceryl MonoRicinoleate...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes de chimie organique › Isomérie › Identification of Glyceryl MonoRicinoleate...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Carboxylic ester hydrolases › Triacylglycerol lipase › Identification of Glyceryl MonoRicinoleate...
questionsmedicales.fr › Lipides › Acides gras › Acides gras insaturés › Acides gras monoinsaturés › Acides oléiques › Acide ricinoléique › Identification of Glyceryl MonoRicinoleate...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Rosanae › Malpighiales › Euphorbiaceae › Ricinus › Identification of Glyceryl MonoRicinoleate...