Multi-institutional experience of genetic diagnosis in Ecuador: National registry of chromosome alterations and polymorphisms.


Journal

Molecular genetics & genomic medicine
ISSN: 2324-9269
Titre abrégé: Mol Genet Genomic Med
Pays: United States
ID NLM: 101603758

Informations de publication

Date de publication:
02 2020
Historique:
received: 02 07 2019
revised: 06 11 2019
accepted: 14 11 2019
pubmed: 13 12 2019
medline: 27 3 2021
entrez: 13 12 2019
Statut: ppublish

Résumé

Detection of chromosomal abnormalities is crucial in various medical areas; to diagnose birth defects, genetic disorders, and infertility, among other complex phenotypes, in individuals across a wide range of ages. Hence, the present study wants to contribute to the knowledge of type and frequency of chromosomal alterations and polymorphisms in Ecuador. Cytogenetic registers from different Ecuadorian provinces have been merged and analyzed to construct an open-access national registry of chromosome alterations and polymorphisms. Of 28,806 karyotypes analyzed, 6,008 (20.9%) exhibited alterations. Down syndrome was the most frequent autosome alteration (88.28%), followed by Turner syndrome (60.50%), a gonosome aneuploidy. A recurrent high percentage of Down syndrome mosaicism (7.45%) reported here, as well as by previous Ecuadorian preliminary registries, could be associated with geographic location and admixed ancestral composition. Translocations (2.46%) and polymorphisms (7.84%) were not as numerous as autosomopathies (64.33%) and gonosomopathies (25.37%). Complementary to conventional cytogenetics tests, molecular tools have allowed identification of submicroscopic alterations regions or candidate genes which can be possibly implicated in patients' symptoms and phenotypes. The Ecuadorian National Registry of Chromosome Alterations and Polymorphisms provides a baseline to better understand chromosomal abnormalities in Ecuador and therefore their clinical management and awareness. This data will guide public policy makers to promote and financially support cytogenetic and genetic testing.

Sections du résumé

BACKGROUND
Detection of chromosomal abnormalities is crucial in various medical areas; to diagnose birth defects, genetic disorders, and infertility, among other complex phenotypes, in individuals across a wide range of ages. Hence, the present study wants to contribute to the knowledge of type and frequency of chromosomal alterations and polymorphisms in Ecuador.
METHODS
Cytogenetic registers from different Ecuadorian provinces have been merged and analyzed to construct an open-access national registry of chromosome alterations and polymorphisms.
RESULTS
Of 28,806 karyotypes analyzed, 6,008 (20.9%) exhibited alterations. Down syndrome was the most frequent autosome alteration (88.28%), followed by Turner syndrome (60.50%), a gonosome aneuploidy. A recurrent high percentage of Down syndrome mosaicism (7.45%) reported here, as well as by previous Ecuadorian preliminary registries, could be associated with geographic location and admixed ancestral composition. Translocations (2.46%) and polymorphisms (7.84%) were not as numerous as autosomopathies (64.33%) and gonosomopathies (25.37%). Complementary to conventional cytogenetics tests, molecular tools have allowed identification of submicroscopic alterations regions or candidate genes which can be possibly implicated in patients' symptoms and phenotypes.
CONCLUSION
The Ecuadorian National Registry of Chromosome Alterations and Polymorphisms provides a baseline to better understand chromosomal abnormalities in Ecuador and therefore their clinical management and awareness. This data will guide public policy makers to promote and financially support cytogenetic and genetic testing.

Identifiants

pubmed: 31830383
doi: 10.1002/mgg3.1087
pmc: PMC7005643
doi:

Types de publication

Journal Article Multicenter Study

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1087

Informations de copyright

© 2019 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc.

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Auteurs

César Paz-Y-Miño (C)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

Verónica Yumiceba (V)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

Germania Moreta (G)

Servicio de Genética Médica, Hospital de Especialidades, Quito, Ecuador.

Rosario Paredes (R)

Servicio de Genética Médica, Hospital de Especialidades, Quito, Ecuador.

Mónica Ruiz (M)

CitoGen, Quito, Ecuador.

Ligia Ocampo (L)

Laboratorio de Genética, Génica Laboratorios, Quito, Ecuador.

Arianne Llamos Paneque (A)

Servicio de Genética Médica, Hospital de Especialidades, Quito, Ecuador.

Catalina Ochoa Pérez (C)

Laboratorio de Citogenética, SOLCA Núcleo de Cuenca, Cuenca, Ecuador.

Juan Carlos Ruiz-Cabezas (JC)

Hospital "Dr. Juan Tanca Marengo", SOLCA Matriz, Guayaquil, Ecuador.
Facultad de Medicina, Universidad Espíritu Santo, Guayaquil, Ecuador.

Jenny Álvarez Vidal (J)

Centro de Diagnóstico y Estudios Biomédicos, Facultad de Ciencia Médicas, Universidad de Cuenca, Cuenca, Ecuador.

Idarmis Jiménez Torres (I)

Hospital Gineco Obstétrico Isidro Ayora, Ministerio de Salud Pública, Quito, Ecuador.

Ramón Vargas-Vera (R)

Facultad de Ciencias Médicas, Universidad de Guayaquil, Guayaquil, Ecuador.

Fernando Cruz (F)

Centro de Genética Médica, CEGEMED, Ministerio de Salud Pública, Quito, Ecuador.

Víctor Hugo Guapi N (VH)

Hospital General Provincial "Luis G. Dávila", Ministerio de Salud Pública, Tulcán, Ecuador.

Martha Montalván (M)

Centro de Investigaciones, Universidad Espíritu Santo, Guayaquil, Ecuador.

Sara Meneses Álvarez (S)

Hospital General Docente Ambato, Ministerio de Salud Pública, Ambato, Ecuador.

Maribel Garzón Castro (M)

Servicio de Genética Médica, Hospital de Especialidades, Quito, Ecuador.

Elizabeth Lamar Segura (E)

Servicio de Genética Médica, Hospital de Especialidades, Quito, Ecuador.

María Augusta Recalde Báez (MA)

Servicio de Genética Médica, Hospital de Especialidades, Quito, Ecuador.

María Elena Naranjo (ME)

Servicio de Genética Médica, Hospital de Especialidades, Quito, Ecuador.

Nina Tambaco Jijón (N)

Servicio de Genética Médica, Hospital de Especialidades, Quito, Ecuador.

María Sinche (M)

Hospital "Dr. Juan Tanca Marengo", SOLCA Matriz, Guayaquil, Ecuador.

Pedro Licuy (P)

Hospital "Dr. Juan Tanca Marengo", SOLCA Matriz, Guayaquil, Ecuador.

Ramiro Burgos (R)

Hospital "Dr. Juan Tanca Marengo", SOLCA Matriz, Guayaquil, Ecuador.

Fabián Porras-Borja (F)

Centro de Genética Médica, CEGEMED, Ministerio de Salud Pública, Quito, Ecuador.

Gabriela Echeverría-Garcés (G)

Centro de Genética Médica, CEGEMED, Ministerio de Salud Pública, Quito, Ecuador.

Andy Pérez-Villa (A)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

Isaac Armendáriz-Castillo (I)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

Jennyfer M García-Cárdenas (JM)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

Santiago Guerrero (S)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

Patricia Guevara-Ramírez (P)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

Andrés López-Cortés (A)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

Ana Karina Zambrano (AK)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

Paola E Leone (PE)

Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador.

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