Homozygous variants in AMPD2 and COL11A1 lead to a complex phenotype of pontocerebellar hypoplasia type 9 and Stickler syndrome type 2.


Journal

American journal of medical genetics. Part A
ISSN: 1552-4833
Titre abrégé: Am J Med Genet A
Pays: United States
ID NLM: 101235741

Informations de publication

Date de publication:
03 2020
Historique:
received: 24 09 2019
revised: 17 10 2019
accepted: 26 11 2019
pubmed: 14 12 2019
medline: 5 1 2021
entrez: 14 12 2019
Statut: ppublish

Résumé

Pontocerebellar hypoplasia type 9 (PCH9) is an autosomal recessive neurodevelopmental disorder caused by pathogenic variants in the AMPD2 gene. We evaluated the son of a consanguineous couple who presented with profound hypotonia and global developmental delay. Other features included sensorineural hearing loss, asymmetric astigmatism, and high myopia. Clinical whole-exome sequence analysis identified a homozygous missense variant in AMPD2 (NM_001257360.1:c.2201C > T, p.[Pro734Leu]) that has not been previously reported. Given the strong phenotypic overlap with PCH9, including the identification of the typical "Figure 8" appearance of the brainstem on neuroimaging, we suspect this variant was causative of the neurodevelopmental disability in this individual. An additional homozygous nonsense variant in COL11A1 (NM_001854.4:c.1168G > T, p.[Glu390Ter]) was identified. Variants in this alternatively spliced region of COL11A1 have been identified to cause an autosomal recessive form of Stickler syndrome type 2 characterized by sensorineural hearing loss and eye abnormalities, but without musculoskeletal abnormalities. The COL11A1 variant likely also contributed to the individual's phenotype, suggesting two potentially relevant genetic findings. This challenging case highlights the importance of detailed phenotypic characterization when interpreting whole exome data.

Identifiants

pubmed: 31833174
doi: 10.1002/ajmg.a.61452
doi:

Substances chimiques

COL11A1 protein, human 0
Collagen Type XI 0
AMP Deaminase EC 3.5.4.6
AMPD2 protein, human EC 3.5.4.6

Types de publication

Case Reports Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

557-560

Informations de copyright

© 2019 Wiley Periodicals, Inc.

Références

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Akizu, N., Cantagrel, V., Schroth, J., Cai, N., Vaux, K., McCloskey, D., … Gleeson, J. G. (2013). AMPD2 regulates GTP synthesis and is mutated in a potentially treatable neurodegenerative brainstem disorder. Cell, 154(3), 505-517.
Alzahrani, F., Alshammari, M. J., & Alkuraya, F. S. (2012, December 15). Molecular pathogenesis of fibrochondrogenesis: Is it really simple COL11A1 deficiency? Gene, 511, 480-481.
Kortüm, F., Jamra, R. A., Alawi, M., Berry, S. A., Borck, G., Helbig, K. L., … Kutsche, K. (2018). Clinical and genetic spectrum of AMPD2-related pontocerebellar hypoplasia type 9. European Journal of Human Genetics, 26(5), 695-708.
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Marsh, A. P. L., Yap, P., Tan, T., Pope, K., White, S. M., Chong, B., … Lockhart, P. J. (2017). A novel AMPD2 mutation outside the AMP deaminase domain causes pontocerebellar hypoplasia type 9. American Journal of Medical Genetics Part A, 173(3), 820-823.
Morris, N. P., Oxford, J. T., Davies, G. B. M., Smoody, B. F., & Keene, D. R. (2000). Developmentally regulated alternative splicing of the α1(XI) collagen chain: Spatial and temporal segregation of isoforms in the cartilage of fetal rat long bones. Journal of Histochemistry and Cytochemistry, 48(6), 725-741.
Richards, A. J., Fincham, G. S., McNinch, A., Hill, D., Poulson, A. V., Castle, B., … Snead, M. P. (2013). Alternative splicing modifies the effect of mutations in COL11A1 and results in recessive type 2 stickler syndrome with profound hearing loss. Journal of Medical Genetics, 50(11), 765-771.
Richards, S., Aziz, N., Bale, S., Bick, D., Das, S., Gastier-Foster, J., … Rehm, H. L. (2015). Standards and guidelines for the interpretation of sequence variants: A joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genetics in Medicine, 17(5), 405-424.
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Auteurs

Nicolas J Abreu (NJ)

The Center for Gene Therapy, Columbus, Ohio.
Department of Pediatrics, The Ohio State University, Columbus, Ohio.

Daniel C Koboldt (DC)

The Institute for Genomic Medicine at Nationwide Children's Hospital, Columbus, Ohio.
Department of Pediatrics, The Ohio State University, Columbus, Ohio.

Julie M Gastier-Foster (JM)

The Institute for Genomic Medicine at Nationwide Children's Hospital, Columbus, Ohio.
Department of Pediatrics, The Ohio State University, Columbus, Ohio.
Department of Pathology, The Ohio State University, Columbus, Ohio.

Ashita Dave-Wala (A)

The Institute for Genomic Medicine at Nationwide Children's Hospital, Columbus, Ohio.

Kevin M Flanigan (KM)

The Center for Gene Therapy, Columbus, Ohio.
Department of Pediatrics, The Ohio State University, Columbus, Ohio.
Department of Neurology, The Ohio State University, Columbus, Ohio.

Megan A Waldrop (MA)

The Center for Gene Therapy, Columbus, Ohio.
Department of Pediatrics, The Ohio State University, Columbus, Ohio.
Department of Neurology, The Ohio State University, Columbus, Ohio.

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