Base Sequence
Carrier Proteins
Cryoelectron Microscopy
DNA, Bacterial
/ metabolism
DNA-Directed RNA Polymerases
/ metabolism
Escherichia coli
/ metabolism
Escherichia coli Proteins
/ chemistry
Models, Molecular
Mutagenesis, Site-Directed
Nucleic Acid Conformation
Promoter Regions, Genetic
Protein Conformation
RNA, Bacterial
/ metabolism
Transcription Factors
/ chemistry
Transcription Initiation, Genetic
/ physiology
Transcriptional Activation
E. coli
RNA polymerase
TraR
biochemistry
chemical biology
cryo-electron microscopy
molecular biophysics
structural biology
transcription initiation
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
16 12 2019
16 12 2019
Historique:
received:
16
06
2019
accepted:
13
12
2019
pubmed:
17
12
2019
medline:
27
5
2020
entrez:
17
12
2019
Statut:
epublish
Résumé
TraR and its homolog DksA are bacterial proteins that regulate transcription initiation by binding directly to RNA polymerase (RNAP) rather than to promoter DNA. Effects of TraR mimic the combined effects of DksA and its cofactor ppGpp, but the structural basis for regulation by these factors remains unclear. Here, we use cryo-electron microscopy to determine structures of
Identifiants
pubmed: 31841111
doi: 10.7554/eLife.49375
pii: 49375
pmc: PMC6970531
doi:
pii:
Substances chimiques
Carrier Proteins
0
DNA, Bacterial
0
Escherichia coli Proteins
0
RNA, Bacterial
0
TraR protein, E coli
0
Transcription Factors
0
dksA protein, E coli
0
DNA-Directed RNA Polymerases
EC 2.7.7.6
Banques de données
PDB
['6N57', '6N58', '6P1K', '6OUL', '4LJZ', '4LK1', '5W1S', '4YG2']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM114450
Pays : United States
Organisme : NIH HHS
ID : R35 GM118130
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118130
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM037048
Pays : United States
Organisme : NIH HHS
ID : R01 GM37048
Pays : United States
Organisme : NIH HHS
ID : R01 GM114450
Pays : United States
Informations de copyright
© 2019, Chen et al.
Déclaration de conflit d'intérêts
JC, SG, CC, AC, EC, RG, WR, SD No competing interests declared
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