Improved packing of preparative biochromatography columns by mechanical vibration.
bed compaction
column packing method
mechanical vibration
preparative biochromatography
ultrasound-assisted sedimentation
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
19
08
2019
revised:
22
11
2019
accepted:
09
12
2019
pubmed:
18
12
2019
medline:
6
8
2021
entrez:
18
12
2019
Statut:
ppublish
Résumé
The bioprocessing industry relies on packed-bed column chromatography as its primary separation process to attain the required high product purities and fulfill the strict requirements from regulatory bodies. Conventional column packing methods rely on flow packing and/or mechanical compression. In this work, the application of ultrasound and mechanical vibration during packing was studied with respect to packing density and homogeneity. We investigated two widely used biochromatography media, incompressible ceramic hydroxyapatite, and compressible polymethacrylate-based particles, packed in a laboratory-scale column with an inner diameter of 50 mm. It was shown that ultrasonic irradiation led to reduced particle segregation during sedimentation of a homogenized slurry of polymethacrylate particles. However, the application of ultrasound did not lead to an improved microstructure of already packed columns due to the low volumetric energy input (~152 W/L) caused by high acoustic reflection losses. In contrast, the application of pneumatic mechanical vibration led to considerable improvements. Flow-decoupled axial linear vibration was most suitable at a volumetric force output of ~1,190 N/L. In the case of the ceramic hydroxyapatite particles, a 13% further decrease of the packing height was achieved and the reduced height equivalent to a theoretical plate (rHETP) was decreased by 44%. For the polymethacrylate particles, a 18% further packing consolidation was achieved and the rHETP was reduced by 25%. Hence, it was shown that applying mechanical vibration resulted in more efficiently packed columns. The application of vibration furthermore is potentially suitable for in situ elimination of flow channels near the column wall.
Substances chimiques
Silicon Dioxide
7631-86-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2950Subventions
Organisme : German Federation of Industrial Research Associations
ID : IGF 18146 N
Pays : International
Informations de copyright
© 2019 The Authors. Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers.
Références
Guiochon G, Beaver LA. Separation science is the key to successful biopharmaceuticals. J Chromatogr A. 2011;1218(49):8836-8858.
Guiochon G. Monolithic columns in high-performance liquid chromatography. J Chromatogr A. 2007;1168(1-2):101-168.
Cummings LJ, Snyder MA, Brisack K. Protein chromatography on hydroxyapatite columns. Guide to Protein Purification. 2nd ed. Cambdrige, Massachusetts: Academic Press; 2009:387-404.
Gagnon P. Monoclonal antibody purification with hydroxyapatite. N Biotechnol. 2009;25(5):287-293.
Siu SC, Chia C, Mok Y, Pattnaik P. Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method. Biotechnol Prog. 2014;30(6):1319-1325.
Bruns S, Tallarek U. Physical reconstruction of packed beds and their morphological analysis: Core-shell packings as an example. J Chromatogr A. 2011;1218:1849-1860.
Godinho JM, Reising AE, Tallarek U, Jorgenson JW. Implementation of high slurry concentration and sonication to pack high-efficiency, meter-long capillary ultra-high pressure liquid chromatography columns. J Chromatogr A. 2016;1462:165-169.
Günter J, Sofer G, Hagel L. Column packing. Handbook of Process Chromatography: Development, Manufacturing, Validation and Economics. Cambridge: Academic Press; 2007:321-330.
Guiochon G, Sarker M. Consolidation of the packing material in chromatographic columns under dynamic axial compression. I. Fundamental study. J Chromatogr A. 1995;704:247-268.
Schure MR, Maier RS. How does column packing microstructure affect column efficiency in liquid chromatography? J Chromatogr A. 2006;1126:58-69.
Reising AE, Godinho JM, Hormann K, Jorgenson JW, Tallarek U. Larger voids in mechanically stable, loose packings of 1.3 μm frictional, cohesive particles: their reconstruction, statistical analysis, and impact on separation efficiency. J Chromatogr A. 2016;1436:118-132.
Hekmat D, Mornhinweg R, Bloch G, et al. Macroscopic investigation of the transient hydrodynamic memory behavior of preparative packed chromatography beds. J Chromatogr A. 2011;1218:944-950.
Khirevich S, Höltzel A, Seidel-Morgenstern A, Tallarek U. Geometrical and topological measures for hydrodynamic dispersion in confined sphere packings at low column-to-particle diameter ratios. J Chromatogr A. 2012;1262:77-91.
Gritti F, Guiochon G. The current revolution in column technology: how it began, where is it going? J Chromatogr A. 2012;1228:2-19.
Schmidt-Traub H, Schulte M, Seidel-Morgenstern A. Preparative Chromatography. Weinheim, Germany: Wiley-VCH Verlag & Co. KGaA; 2012.
Kong DY, Gerontas S, McCluckie RA, Mewies M, Gruberb D, Titchener-Hooker NJ. Effects of bed compression on protein separation on gel filtration chromatography at bench and pilot scale. Journal of Chemical Technology & Biotechnology. 2017;93(7):1959-1965.
Knox JH, Parcher JF. Effect of column to particle diameter ratio on the dispersion of Unsorbed solutes in chromatography. Anal Chem. 1969;41(12):1599-1606.
Bruns S, Franklin EG, Grinias JP, Godinho JM, Jorgenson JW, Tallarek U. Slurry concentration effects on the bed morphology and separation efficiency of capillaries packed with sub-2 μm particles. J Chromatogr A. 2013;1318:189-197.
Kirkland J, DeStefano J. The art and science of forming packed analytical high-performance liquid chromatography columns. J Chromatogr A. 2006;1126(1-2):50-57.
Martinez A, Kuhn M, Briesen H, Hekmat D. Enhancing the X-ray contrast of polymeric biochromatography particles for three-dimensional imaging. J Chromatogr A. 2019;1590:65-72.
Reising AE, Godinho JM, Jorgenson JW, Tallarek U. Bed morphological features associated with an optimal slurry concentration for reproducible preparation of efficient capillary ultrahigh pressure liquid chromatography columns. J Chromatogr A. 2017;1504:71-82.
Reising AE, Schlabach S, Baranau V, Stoeckel D, Tallarek U. Analysis of packing microstructure and wall effects in a narrow-bore ultrahigh pressure liquid chromatography column using focused ion-beam scanning electron microscopy. J Chromatogr A. 2017;1513:172-182.
Dorn M, Hekmat D. Simulation of the dynamic packing behavior of preparative chromatography columns via discrete particle modeling. Biotechnol Prog. 2016;32(2):363-371.
Jaeger HM, Nagel SR, Behringer RP. Granular solids, liquids, and gases. Review of Modern Physics. 1996;68(4):1259-1273.
Hanotin C, Kiesgen de Richter S, Marchal P, Michot LJ, Baravian C. Vibration-induced liquefaction of granular suspensions. Phys Rev Lett. 2012;108(19):198301.
Lozano C, Luma G, Zuriguel I, Hidalgo RC, Garcimartín A. Breaking arches with vibrations: the role of defects. Phys Rev Lett. 2012;109(6):068001.
Cao YX, Chakrabortty B, Barker GC, Mehta A, Wang YJ. Bridges in three-dimensional granular packings: experiments and simulations. Europhysics Letters. 2013;102(2):24004.
Zorica J, Darko V. Compaction dynamics of vibrated granular materials. Scientific Technical Review. 2012;62(3):39-44.
Chen K, Cole J, Conger C, et al. Packing grains by thermal cycling. Nature. 2006;442(7100):257-257.
Jia X, Brunet T, Laurent J. Elastic weakening of a dense granular pack by acoustic fluidization: slipping, compaction, and aging. Physical Review E. 2011;84(2):020301.
Jia X, Caroli C, Velicky B. Ultrasound propagation in externally stressed granular media. Phys Rev Lett. 1999;82(9):1863-1866.
Tariot A, Gauthier G, Gondret P, et al. Granular compaction by fluidization. EPJ Web of Conferences. 2017;140:100003.
Reichhardt CJO, Lopatina LM, Jia X, Johnson P. Softening of granular packings with dynamic forcing. Physical Review E. 2015;92(2):022203.
Dorn M, Eschbach F, Hekmat D, Weuster-Botz D. Influence of different packing methods on the hydrodynamic stability of chromatography columns. J Chromatogr A. 2017;1516:89-101.
Feeney A, Sikaneta S, Harkness P, Lucas M. An ultrasonic compactor for oil and gas exploration. Physics Procedia. 2016;87:72-78.
Levina M, Rubinstein MH. The effect of ultrasonic vibration on the compaction characteristics of ibuprofen. Drug Dev Ind Pharm. 2002;28(5):495-514.
Levina M, Rubinstein MH. The effect of ultrasonic vibration on the compaction characteristics of paracetamol. J Pharm Sci. 2000;89(6):705-723.
Abedini R, Abdullah A, Alizadeh Y. Ultrasonic assisted hot metal powder compaction. Ultrason Sonochem. 2016;38:704-710.
Fartashvand V, Abdullah A, Sadough Vanini SA. Effects of high power ultrasonic vibration on the cold compaction of titanium. Ultrasonic Sonochemistry. 2017;36:155-161.
Franc M, Vojta J, Sobotnikova J, Pavel C, Zuzana B. Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography. Chemical Papers. 2013;68(1):22-28.
Ehlert S, Rösler T, Tallarek U. Packing density of slurry-packed capillaries at low aspect ratios. J Sep Sci. 2008;31(10):1719-1728.
Shalliker RA, Broyles BS, Guiochon G. Evaluation of the secondary consolidation of columns for liquid chromatography by ultrasonic irradiation. J Chromatogr A. 2000;878(2):153-163.
Ehlert S, Kraiczek K, Mora J-A, Dittmann M, Rozing GP, Tallarek U. Separation efficiency of particle-packed HPLC microchips. Anal Chem. 2008;80(15):5845-5950.
V. Natarajan, A. F. Mann and D. Schubnel, Method of and device for packing a chromatography column. United States of America Patent US20100084342 A1, 8th April 2010.
C. V. Company, “www.clevelandvibrator.com,” Cleveland Vibrator, 2016. https://www.clevelandvibrator.com/images/Documents/Selection%20Guide%20for%20Industrial%20Vibrators.pdf. [Accessed July 19, 2019].
T. Biosciences, “Toyopearl Instruction Manual,” Tosoh Biosciences, https://www.separations.eu.tosohbioscience.com/OpenPDF.aspx?path=/File%20Library/TBG/Products%20Download/Instruction%20Manual/m15p73a.pdf. [Accessed January 2019].
B. Laboratories, “CHT Ceramic Hydroxyapatite Instruction Manual,” BioRad Laboratories, https://www.bio-rad.com/webroot/web/pdf/lsr/literature/LIT611E.PDF. [Accessed January 2019].
Deutsch V, Platte M, Vogt M. Ultraschallprüfung: Grundlagen und industrielle Anwendungen. Berlin: Springer; 1997.
Callens D, Bruneel C, Assaad J. Matching ultrasonic transducer using two matching layers where one of them is glue. NDT & E Int. 2004;37(8):591-596.
Manh T, Nguyen A-TT, Johansen TF, Hoff L. Microfabrication of stacks of acoustic matching layers for 15 MHz ultrasonic transducers. Ultrasonics. 2014;54(2):614-620.
Hekmat D, Kuhn M, Meinhardt V, Weuster-Botz D. Modeling of transient flow through a viscoelastic preparative chromatography packing. Biotechnol Prog. 2013;29(4):958-967.
DePhillips P, Lenhoff AM. Pore size distributions of cation-exchange adsorbents determined by inverse size-exclusion chromatography. J Chromatogr A. 2000;883:39-54.
Aldak GmbH, “Pneumatic vibrators and knockers,” Aldak, 2017. https://www.aldak.com/pneumatic-vibrators/overview.html. [Accessed 29 26 2019].
Lei J, Hill M, Glynne-Jones P. Numerical simulation of 3D boundary-driven acoustic streaming in microfluidic devices. Lab Chip. 2014;14(3):532-541.
Wiklund M, Green R, Ohlin M. Acoustofluidics 14: applications of acoustic streaming in microfluidic devices. Lab Chip. 2012;12:2438-2451.
Bruus H. Acoustofluidics 7: the acoustic radiation force on. Lab Chip. 2012;12:1014-1021.
Hawkes JJ, Radel S. Acoustofluidics 22: multi-wavelength resonators, applications and considerations. Lab Chip. 2013;13(4):610-627.
Carlevaro C, Pugnaloni L. Arches and contact forces in a granular pile. Eur Phys J E. 2012;35(6):44.
Mehta A. Spatial, dynamical and spatiotemporal heterogeneities in granular media. Soft Matter. 2010;6(13):2875-2883.
Sternberg JC. Extracolumn contributions to chromatographic band broadening. Adv Chromatogr. 1966;2:205-270.