Characterization of the binding of cyanidin-3-glucoside to bovine serum albumin and its stability in a beverage model system: A multispectroscopic and chemometrics study.

Animals Anthocyanins / chemistry Beverages Binding Sites Calorimetry, Differential Scanning Cattle Circular Dichroism Glucosides / chemistry Hydrogen Bonding Hydrogen Peroxide / metabolism Microscopy, Atomic Force Models, Molecular Models, Structural Oxidation-Reduction Protein Binding Serum Albumin, Bovine / chemistry Spectrometry, Fluorescence Temperature

Anthocyanin Bovine serum albumin Chemometrics Interaction Stabilization

Journal

Food chemistry

ISSN: 1873-7072

Titre abrégé: Food Chem

Pays: England

ID NLM: 7702639

Informations de publication

Date de publication:
01 May 2020

Historique:

received: 03 06 2019

revised: 30 11 2019

accepted: 03 12 2019

pubmed: 22 12 2019

medline: 14 4 2020

entrez: 22 12 2019

Statut: ppublish

Résumé

Anthocyanins as one of the main natural groups of food colorants undergo quick color fading, which can be diminished through protein association. The stabilization of cyanidin-3-glucoside (CYG) through binding to bovine serum albumin (BSA) was investigated at pH 3.0 using atomic force microscopy and differential scanning calorimetry along with UV-Vis absorption, steady-state fluorescence, circular dichroism, and three-dimensional emission spectral analyses merged with the multivariate curve resolution-alternative least square method. The stabilized CYG molecules were found at the site II of BSA with combined static and dynamic quenching mechanisms. Approximately 93% of the BSA binding sites were occupied in the BSA-CYG complex through hydrogen bonds and van der Waals forces with the binding constant and stoichiometry ratio of 1.88 × 10

Identifiants

DOI: 10.1016/j.foodchem.2019.126015 PMID: 31864188

pubmed: 31864188

pii: S0308-8146(19)32158-2

doi: 10.1016/j.foodchem.2019.126015

pii:

doi:

Substances chimiques

Anthocyanins 0

Glucosides 0

cyanidin-3-O-beta-glucopyranoside 0

Serum Albumin, Bovine 27432CM55Q

Hydrogen Peroxide BBX060AN9V

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

126015

Characterization of the binding of cyanidin-3-glucoside to bovine serum albumin and its stability in a beverage model system: A multispectroscopic and chemometrics study.

Journal

Informations de publication

Résumé

Identifiants

Substances chimiques

Types de publication

Langues

Sous-ensembles de citation

Pagination

Informations de copyright

Auteurs

Seyed Hossein Razavizadegan Jahromi (S)

Reza Farhoosh (R)

Bahram Hemmateenejad (B)

Mehdi Varidi (M)

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Odour generalisation and detection dog training.

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Classifications MeSH