Mass Activated Droplet Sorting (MADS) Enables High-Throughput Screening of Enzymatic Reactions at Nanoliter Scale.
Algorithms
Amines
/ analysis
Enzyme Activation
Enzyme Assays
/ methods
Feasibility Studies
High-Throughput Screening Assays
/ methods
Imidazoles
/ chemistry
Microfluidic Analytical Techniques
Microfluidics
/ methods
Pyridines
/ analysis
Spectrometry, Mass, Electrospray Ionization
Transaminases
/ metabolism
biocatalysis
droplet microfluidics
high-throughput screening
mass spectrometry
microreactors
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
09 03 2020
09 03 2020
Historique:
received:
24
10
2019
revised:
21
11
2019
pubmed:
24
12
2019
medline:
18
3
2021
entrez:
24
12
2019
Statut:
ppublish
Résumé
Microfluidic droplet sorting enables the high-throughput screening and selection of water-in-oil microreactors at speeds and volumes unparalleled by traditional well-plate approaches. Most such systems sort using fluorescent reporters on modified substrates or reactions that are rarely industrially relevant. We describe a microfluidic system for high-throughput sorting of nanoliter droplets based on direct detection using electrospray ionization mass spectrometry (ESI-MS). Droplets are split, one portion is analyzed by ESI-MS, and the second portion is sorted based on the MS result. Throughput of 0.7 samples s
Identifiants
pubmed: 31868984
doi: 10.1002/anie.201913203
doi:
Substances chimiques
Amines
0
Imidazoles
0
Pyridines
0
Transaminases
EC 2.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4470-4477Informations de copyright
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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