Soluble versions of outer membrane cytochromes function as exporters for heterologously produced cargo proteins.
Cellulase
Fusion proteins
Outer membrane cytochromes
Protein export
Shewanella oneidensis
Type II secretion system
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
23 Dec 2019
23 Dec 2019
Historique:
received:
21
10
2019
accepted:
13
12
2019
entrez:
25
12
2019
pubmed:
25
12
2019
medline:
16
4
2020
Statut:
epublish
Résumé
This study reveals that it is possible to secrete truncated versions of outer membrane cytochromes into the culture supernatant and that these proteins can provide a basis for the export of heterologously produced proteins. Different soluble and truncated versions of the outer membrane cytochrome MtrF were analyzed for their suitability to be secreted. A protein version with a very short truncation of the N-terminus to remove the recognition sequence for the addition of a lipid anchor is secreted efficiently to the culture supernatant, and moreover this protein could be further truncated by a deletion of 160 amino acid and still is detectable in the supernatant. By coupling a cellulase to this soluble outer membrane cytochrome, the export efficiency was measured by means of relative cellulase activity. We conclude that outer membrane cytochromes of S. oneidensis can be applied as transporters for the export of target proteins into the medium using the type II secretion pathway.
Identifiants
pubmed: 31870378
doi: 10.1186/s12934-019-1270-2
pii: 10.1186/s12934-019-1270-2
pmc: PMC6929479
doi:
Substances chimiques
Bacterial Outer Membrane Proteins
0
Cytochromes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
216Références
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