Optogenetic stimulation of kisspeptin neurones within the posterodorsal medial amygdala increases luteinising hormone pulse frequency in female mice.
LH
MePD
amygdala
kisspeptin
optogenetics
Journal
Journal of neuroendocrinology
ISSN: 1365-2826
Titre abrégé: J Neuroendocrinol
Pays: United States
ID NLM: 8913461
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
03
10
2018
revised:
25
11
2019
accepted:
19
12
2019
pubmed:
25
12
2019
medline:
13
5
2021
entrez:
25
12
2019
Statut:
ppublish
Résumé
Kisspeptin within the arcuate nucleus of the hypothalamus is a critical neuropeptide in the regulation of reproduction. Together with neurokinin B and dynorphin A, arcuate kisspeptin provides the oscillatory activity that drives the pulsatile secretion of gonadotrophin-releasing hormone (GnRH), and therefore luteinising hormone (LH) pulses, and is considered to be a central component of the GnRH pulse generator. It is well established that the amygdala also exerts an influence over gonadotrophic hormone secretion and reproductive physiology. The discovery of kisspeptin and its receptor within the posterodorsal medial amygdala (MePD) and our recent finding showing that intra-MePD administration of kisspeptin or a kisspeptin receptor antagonist results in increased LH secretion and decreased LH pulse frequency, respectively, suggests an important role for amygdala kisspeptin signalling in the regulation of the GnRH pulse generator. To further investigate the function of amygdala kisspeptin, the present study used an optogenetic approach to selectively stimulate MePD kisspeptin neurones and examine the effect on pulsatile LH secretion. MePD kisspeptin neurones in conscious Kiss1-Cre mice were virally infected to express the channelrhodopsin 2 protein and selectively stimulated by light via a chronically implanted fibre optic cannula. Continuous stimulation using 5 Hz resulted in an increased LH pulse frequency, which was not observed at the lower stimulation frequencies of 0.5 and 2 Hz. In wild-type animals, continuous stimulation at 5 Hz did not affect LH pulse frequency. These results demonstrate that selective activation of MePD Kiss1 neurones can modulate hypothalamic GnRH pulse generator frequency.
Identifiants
pubmed: 31872920
doi: 10.1111/jne.12823
pmc: PMC7116078
mid: EMS94135
doi:
Substances chimiques
Kiss1 protein, mouse
0
Kisspeptins
0
Luteinizing Hormone
9002-67-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12823Subventions
Organisme : Medical Research Council
ID : MR/N022637/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S000550/1
Pays : United Kingdom
Informations de copyright
© 2019 The Authors. Journal of Neuroendocrinology published by John Wiley & Sons Ltd on behalf of British Society for Neuroendocrinology.
Références
Endocrinology. 2011 May;152(5):2020-30
pubmed: 21363930
PLoS One. 2017 Aug 28;12(8):e0183596
pubmed: 28846730
J Clin Invest. 2017 Feb 1;127(2):709-719
pubmed: 28112678
Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):2413-2418
pubmed: 28196880
Psychoneuroendocrinology. 2015 Jan;51:307-17
pubmed: 25462903
Brain Res. 1998 Jun 15;796(1-2):132-42
pubmed: 9689463
Am J Physiol. 1966 Nov;211(5):1103-7
pubmed: 5924030
J Neuroendocrinol. 2005 Jan;17(1):22-8
pubmed: 15720472
Neurosci Res. 1999 Mar;33(3):187-94
pubmed: 10211762
Elife. 2016 Aug 23;5:
pubmed: 27549338
J Neuroendocrinol. 2011 Oct;23(10):863-70
pubmed: 21815953
Endocrinology. 2016 Dec;157(12):4794-4802
pubmed: 27715255
N Engl J Med. 2003 Oct 23;349(17):1614-27
pubmed: 14573733
Endocrinology. 2008 Aug;149(8):3926-32
pubmed: 18450966
J Neurosci. 2015 Oct 28;35(43):14533-43
pubmed: 26511244
Am J Physiol. 1970 Mar;218(3):622-6
pubmed: 4905480
J Neurosci. 2014 Jun 25;34(26):8699-715
pubmed: 24966371
Neuroscience. 2006 Jul 7;140(3):791-800
pubmed: 16650943
Endocrinology. 2011 Feb;152(2):545-55
pubmed: 21159851
J Neuroendocrinol. 2016 Nov;28(11):
pubmed: 27663274
Neuroendocrinology. 2017;104(3):223-238
pubmed: 27054958
J Neurosci. 2019 Dec 4;39(49):9738-9747
pubmed: 31645462
Neuron. 2005 May 19;46(4):647-60
pubmed: 15944132
Front Neuroanat. 2012 Aug 21;6:33
pubmed: 22933993
PLoS One. 2019 Mar 27;14(3):e0213927
pubmed: 30917148
Elife. 2018 Dec 19;7:
pubmed: 30565563
Neuroscience. 2011 Jan 26;173:37-56
pubmed: 21093546
Endocrinology. 2017 Nov 1;158(11):3914-3928
pubmed: 28938486
J Neuroendocrinol. 2018 Mar;30(3):e12572
pubmed: 29356147
Prog Neuropsychopharmacol Biol Psychiatry. 2005 Dec;29(8):1201-13
pubmed: 16271821
Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6496-9
pubmed: 9177246
Physiol Behav. 2012 Jan 18;105(2):554-9
pubmed: 21945865
J Neurosci. 2014 Dec 3;34(49):16296-308
pubmed: 25471569
Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):E10216-E10223
pubmed: 29109258
PLoS One. 2012;7(7):e39001
pubmed: 22802933
Endocrinology. 2013 Dec;154(12):4939-45
pubmed: 24092638
Neuroscience. 2001;104(4):1085-97
pubmed: 11457592
Brain Struct Funct. 2016 May;221(4):2035-47
pubmed: 25758403
PLoS One. 2018 Jan 29;13(1):e0191794
pubmed: 29377906
Neuroendocrinology. 2019;108(3):172-189
pubmed: 30537700
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10972-6
pubmed: 12944565