WBP11 is required for splicing the TUBGCP6 pre-mRNA to promote centriole duplication.
Cell Cycle
Centrioles
/ genetics
Centrosome
/ physiology
Colonic Neoplasms
/ genetics
DNA-Binding Proteins
/ genetics
HCT116 Cells
HeLa Cells
Humans
Introns
Microtubule-Associated Proteins
/ genetics
Mitosis
Nuclear Receptor Coactivators
/ genetics
RNA Precursors
/ genetics
RNA Splicing
/ genetics
RNA Splicing Factors
/ genetics
RNA, Messenger
/ genetics
Transcriptome
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
06 01 2020
06 01 2020
Historique:
received:
30
04
2019
revised:
24
09
2019
accepted:
22
10
2019
entrez:
25
12
2019
pubmed:
25
12
2019
medline:
28
7
2020
Statut:
ppublish
Résumé
Centriole duplication occurs once in each cell cycle to maintain centrosome number. A previous genome-wide screen revealed that depletion of 14 RNA splicing factors leads to a specific defect in centriole duplication, but the cause of this deficit remains unknown. Here, we identified an additional pre-mRNA splicing factor, WBP11, as a novel protein required for centriole duplication. Loss of WBP11 results in the retention of ∼200 introns, including multiple introns in TUBGCP6, a central component of the γ-TuRC. WBP11 depletion causes centriole duplication defects, in part by causing a rapid decline in the level of TUBGCP6. Several additional splicing factors that are required for centriole duplication interact with WBP11 and are required for TUBGCP6 expression. These findings provide insight into how the loss of a subset of splicing factors leads to a failure of centriole duplication. This may have clinical implications because mutations in some spliceosome proteins cause microcephaly and/or growth retardation, phenotypes that are strongly linked to centriole defects.
Identifiants
pubmed: 31874114
pii: 133543
doi: 10.1083/jcb.201904203
pmc: PMC7039186
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
Microtubule-Associated Proteins
0
Nuclear Receptor Coactivators
0
RNA Precursors
0
RNA Splicing Factors
0
RNA, Messenger
0
SNW1 protein, human
0
TUBGCP6 protein, human
0
WBP11 protein, human
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM114119
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007445
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM133897
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM122846
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM135457
Pays : United States
Informations de copyright
© 2019 Park et al.
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