Electrolysis phenomena in electrophoresis.
Background electrolyte
Capillary zone electrophoresis
Contactless conductivity detection
Electrolysis
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
23
10
2019
revised:
25
11
2019
accepted:
20
12
2019
pubmed:
29
12
2019
medline:
16
3
2021
entrez:
29
12
2019
Statut:
ppublish
Résumé
We present a new theoretical approach for calculating changes in the physico-chemical properties of BGEs for measurements by CZE due to the electrolysis in electrode vials (vessels). Electrolysis is an inevitable phenomenon in any measurement in CZE. Water electrolysis, which occurs in most measurements, can significantly alter the composition of the BGE in electrode vials and in the separation capillary and has a negative influence on the robustness and quality of separations. The ability to predict changes in the composition of the BGE is important for evaluation of the suitability of the BGEs for repeating electrophoretic runs. We compared theoretically calculated changes in the physico-chemical properties (pH, conductivity) with those measured using pH-microelectrode and contactless conductivity detection of the BGE after the electrophoretic run. We confirmed the validity of our theoretical approach with a common BGE composed of acid-base pair, where one constituent is fully dissociated while the second constituent is dissociated by only half, and with Good's buffer. As predicted by theoretical approach, the changes in the physico-chemical properties of the Good's buffer after the electrophoretic run were several times lower than in the case of a common BGE composed of a weak acid - strong base pair.
Identifiants
pubmed: 31883353
doi: 10.1002/elps.201900411
doi:
Substances chimiques
Buffers
0
Electrolytes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
536-544Subventions
Organisme : Czech Science Foundation
ID : 18-11776S
Pays : International
Organisme : Agilent Foundation
ID : No. 4135
Pays : International
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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