Molecular Wiring of a Mitochondrial Translational Feedback Loop.
Cytochromes b
/ biosynthesis
Gene Expression Regulation
Membrane Proteins
/ metabolism
Mitochondria
/ genetics
Mitochondrial Proteins
/ genetics
Molecular Chaperones
/ metabolism
Protein Biosynthesis
RNA, Messenger
/ analysis
RNA-Binding Proteins
/ genetics
Ribosomes
/ metabolism
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
Trans-Activators
/ metabolism
Mitochondrial biogenesis
mitochondrial gene expression
mitochondrial ribosome
mitoribosome interactors
respiratory chain assembly
ribosomal tunnel exit
translation activation
translation regulation
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
20 02 2020
20 02 2020
Historique:
received:
26
02
2019
revised:
03
09
2019
accepted:
25
11
2019
pubmed:
31
12
2019
medline:
15
9
2020
entrez:
30
12
2019
Statut:
ppublish
Résumé
The mitochondrial oxidative phosphorylation system comprises complexes assembled from subunits derived from mitochondrial and nuclear gene expression. Both genetic systems are coordinated by feedback loops, which control the synthesis of specific mitochondrial encoded subunits. Here, we studied how this occurs in the case of cytochrome b, a key subunit of mitochondrial complex III. Our data suggest the presence of a molecular rheostat consisting of two translational activators, Cbp3-Cbp6 and Cbs1, which operates at the mitoribosomal tunnel exit to connect translational output with assembly efficiency. When Cbp3-Cbp6 is engaged in assembly of cytochrome b, Cbs1 binds to the tunnel exit to sequester the cytochrome b-encoding mRNA, repressing its translation. After mediating complex III assembly, binding of Cbp3-Cbp6 to the tunnel exit replaces Cbs1 and the bound mRNA to permit cytochrome b synthesis. Collectively, the data indicate the molecular wiring of a feedback loop to regulate synthesis of a mitochondrial encoded protein.
Identifiants
pubmed: 31883951
pii: S1097-2765(19)30867-6
doi: 10.1016/j.molcel.2019.11.019
pii:
doi:
Substances chimiques
CBP3 protein, S cerevisiae
0
CBS1 protein, S cerevisiae
0
CBS2 protein, S cerevisiae
0
Cbp6 protein, S cerevisiae
0
Membrane Proteins
0
Mitochondrial Proteins
0
Molecular Chaperones
0
RNA, Messenger
0
RNA-Binding Proteins
0
Saccharomyces cerevisiae Proteins
0
Trans-Activators
0
cbp1 protein, S cerevisiae
0
Cytochromes b
9035-37-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
887-900.e5Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.