Identification and functional characterization of methyl-CpG binding domain protein from Tribolium castaneum.
DNA methylation
Methyl-CpG binding domain protein
Neuronal deficiency
RNAi
Tribolium castaneum
Journal
Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
13
06
2019
revised:
03
12
2019
accepted:
25
12
2019
pubmed:
31
12
2019
medline:
7
4
2021
entrez:
30
12
2019
Statut:
ppublish
Résumé
Methyl-CpG binding domain proteins (MBD) can specifically bind to methylated CpG sites and play important roles in epigenetic gene regulation. Here, we identified and functionally characterized the MBD protein in Tribolium castaneum. T. castaneum genome encodes only one MBD protein: TcMBD2/3. RNA interference targeting this gene at different developmental stages caused lethal phenotypes including metamorphosis deficiency in larvae and pupae, gastrointestinal system problems and fecundity deficiency in adult. Moreover, Tcmbd2/3 knockdown adult showed progressive reduced locomoter activity, a typical neurodegeneration phenotype. This is a common feature of DNA methylation in mammals and has not been found in other insects. However, band shift assays demonstrated that TcMBD2/3 could not bind to methylated DNA, indicating the essential roles of TcMBD2/3 is independent of DNA methylation. Our study provides Tcmbd2/3 plays important roles in T. castaneum and gives new insights into the potential mechanism of action of MBD proteins in insect.
Identifiants
pubmed: 31884160
pii: S0888-7543(19)30353-2
doi: 10.1016/j.ygeno.2019.12.018
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
Insect Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2223-2232Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.