Molecular characterization and expression analysis of two crucial MAPKs- jnk1 and erk1 as cellular signal transducers in Labeo rohita in response to PAMPs stimulation and pathogenic invasion.
Adjuvants, Immunologic
/ pharmacology
Aeromonas hydrophila
/ physiology
Animals
Cyprinidae
/ genetics
Fish Diseases
/ enzymology
Fish Proteins
/ genetics
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
/ drug effects
Gram-Negative Bacterial Infections
/ enzymology
JNK Mitogen-Activated Protein Kinases
/ genetics
Mitogen-Activated Protein Kinase 3
/ genetics
Pathogen-Associated Molecular Pattern Molecules
/ immunology
Poly I-C
/ pharmacology
Signal Transduction
/ genetics
Labeo rohita
erk1
jnk1
MAPK
Journal
Journal of fish biology
ISSN: 1095-8649
Titre abrégé: J Fish Biol
Pays: England
ID NLM: 0214055
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
06
09
2019
accepted:
27
12
2019
pubmed:
31
12
2019
medline:
26
6
2020
entrez:
31
12
2019
Statut:
ppublish
Résumé
Mitogen-activated protein kinases (MAPKs) are crucial Ser/Thr protein kinases that play important roles in innate immunity by converting extracellular stimuli into a wide range of cellular responses, including the production of cytokines. In this study, two MAPK genes, jnk1 and erk1, were cloned and characterized in rohu (Labeo rohita), a commercially important freshwater fish species in the Indian subcontinent. In healthy rohu, both jnk1 and erk1 gene expressions were highest in the spleen as compared to gill, liver, blood and kidney tissues. In vitro stimulation of the L. rohita gill (LRG) cell line with γ-D-glutamyl-meso-diaminopimelic acid, muramyl dipeptide and polyinosinic: polycytidylic acid (poly I:C) resulted in significantly enhanced expressions of jnk1 and erk1 genes. In the in vivo experiments, jnk1 and erk1 gene expressions were also enhanced in lipopolysaccharides and poly I:C-treatment. Infection of rohu fingerlings with Aeromonas hydrophila and Bacillus subtilis revealed significantly enhanced expressions of the jnk1 and erk1 genes in all of the tested organs/tissues. Together these results imply the important role of jnk1 and erk1 genes in fish during pathogenic invasion and diseases.
Substances chimiques
Adjuvants, Immunologic
0
Fish Proteins
0
Pathogen-Associated Molecular Pattern Molecules
0
JNK Mitogen-Activated Protein Kinases
EC 2.7.11.24
Mitogen-Activated Protein Kinase 3
EC 2.7.11.24
Poly I-C
O84C90HH2L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
580-589Subventions
Organisme : This work was supported by a grant from the National Agricultural Science Fund (NASF) of the Indian Council of Agricultural Research (ICAR) (project code: NASF/ BS-4003).
Informations de copyright
© 2019 The Fisheries Society of the British Isles.
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