Chlamydiaceae in wild, feral and domestic pigeons in Switzerland and insight into population dynamics by Chlamydia psittaci multilocus sequence typing.
Animals
Animals, Domestic
Animals, Wild
Bacterial Outer Membrane Proteins
/ chemistry
Bird Diseases
/ diagnosis
Chlamydiaceae
/ classification
Chlamydophila psittaci
/ genetics
Columbidae
DNA, Bacterial
/ chemistry
Multilocus Sequence Typing
Phylogeny
Population Dynamics
Psittacosis
/ diagnosis
RNA, Ribosomal, 16S
/ chemistry
Switzerland
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
17
07
2019
accepted:
18
11
2019
entrez:
31
12
2019
pubmed:
31
12
2019
medline:
27
3
2020
Statut:
epublish
Résumé
Feral pigeons, common wood pigeons and Eurasian collared doves are the most common representatives of the Columbidae family in Switzerland and are mostly present in highly populated, urban areas. Pigeons may carry various members of the obligate intracellular Chlamydiaceae family, particularly Chlamydia (C.) psittaci, a known zoonotic agent, and C. avium. The objective of the study was to identify the infection rates of common free-roaming pigeons for different Chlamydia species with the overall aim to assess the risk pigeons pose to public health. In this study, 431 pigeons (323 feral pigeons, 34 domestic pigeons, 39 Eurasian collared doves, 35 common wood pigeons) from several geographic locations in Switzerland were investigated for the presence of Chlamydiaceae. Samples consisted of pooled choanal-cloacal swabs (n = 174), liver samples (n = 52), and paired swab and liver samples from 205 pigeons (n = 410). All 636 samples were screened using a Chlamydiaceae family-specific 23S rRNA real-time PCR (qPCR). Subsequent species identification was performed by DNA-microarray assay, sequencing of a 16S rRNA gene fragment and a C. psittaci specific qPCR. In total, 73 of the 431 pigeons tested positive for Chlamydiaceae, of which 68 were positive for C. psittaci, four were C. avium-positive and one pigeon was co-infected with C. avium and C. psittaci. The highest infection rates were detected in feral (64/323) and domestic pigeons (5/34). Common wood pigeons (2/35) and Eurasian collared doves (2/39) revealed lower infection rates. Additionally, multilocus sequence typing of twelve selected C. psittaci-positive samples revealed closely related sequence types (ST) between and within different Swiss cities. Furthermore, liver and corresponding swab samples from the same bird were colonized by the same ST. Considering the high infection rates of C. psittaci in domestic and feral pigeons, close or frequent contact to these birds poses a human health risk.
Identifiants
pubmed: 31887111
doi: 10.1371/journal.pone.0226088
pii: PONE-D-19-20000
pmc: PMC6936790
doi:
Substances chimiques
Bacterial Outer Membrane Proteins
0
DNA, Bacterial
0
RNA, Ribosomal, 16S
0
OMPA outer membrane proteins
149024-69-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0226088Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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