Synthesis of Single-Stranded RNA Probes by In Vitro Transcription.
Journal
Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530
Informations de publication
Date de publication:
02 01 2020
02 01 2020
Historique:
entrez:
4
1
2020
pubmed:
4
1
2020
medline:
29
7
2021
Statut:
epublish
Résumé
In this protocol, we describe procedures for synthesizing RNA probes of high specific activity from DNA templates containing promoters for bacteriophage-encoded RNA polymerases. The DNA fragment to be transcribed should have previously been cloned into one of the commercially available plasmids containing bacteriophage RNA polymerase promoters. Alternatively, DNA templates can be synthesized in polymerase chain reactions (PCRs) using gene-specific primers whose 5' ends encode synthetic promoters for bacteriophage-encoded RNA polymerases.
Identifiants
pubmed: 31896610
pii: 2020/1/pdb.prot100628
doi: 10.1101/pdb.prot100628
doi:
Substances chimiques
DNA, Viral
0
RNA Probes
0
RNA
63231-63-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
100628Informations de copyright
© 2020 Cold Spring Harbor Laboratory Press.