Spectral editing of intra- and inter-chain methyl-methyl NOEs in protein complexes.
Assignment
Isotopic labeling
Large protein
Long range NOE
Methyl groups
NMR
Protein complex
Structure determination
Journal
Journal of biomolecular NMR
ISSN: 1573-5001
Titre abrégé: J Biomol NMR
Pays: Netherlands
ID NLM: 9110829
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
07
10
2019
accepted:
10
12
2019
pubmed:
4
1
2020
medline:
10
10
2020
entrez:
4
1
2020
Statut:
ppublish
Résumé
Specific isotopic labeling of methyl groups in a perdeuterated protein background enables the detection of long range NOEs in proteins or high molecular weight complexes. We introduce here an approach, combining an optimized isotopic labeling scheme with a specifically tailored NMR pulse sequence, to distinguish between intramolecular and intermolecular NOE connectivities. In hetero-oligomeric complexes, this strategy enables sign encoding of intra-subunit and inter-subunit NOEs. For homo-oligomeric assemblies, our strategy allows the specific detection of intra-chain NOEs in high resolution 3D NOESY spectra. The general principles, possibilities and limitations of this approach are presented. Applications of this approach for the detection of intermolecular NOEs in a hetero-hexamer, and the assignment of methyl
Identifiants
pubmed: 31897934
doi: 10.1007/s10858-019-00293-x
pii: 10.1007/s10858-019-00293-x
doi:
Substances chimiques
Molecular Chaperones
0
Multiprotein Complexes
0
Proteins
0
prefoldin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
83-94Subventions
Organisme : ANR
ID : ANR-10-INBS-05-02
Organisme : CBH-EUR-GS
ID : ANR-17-EURE-0003
Organisme : Idex
ID : ANR-15-IDEX-02
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