Can Direct-Acting Antiviral Treatment Change the Immunologic Risk Profile in Patients Infected with Hepatitis C Virus Who Are on the Cadaveric Waiting List?


Journal

Transplantation proceedings
ISSN: 1873-2623
Titre abrégé: Transplant Proc
Pays: United States
ID NLM: 0243532

Informations de publication

Date de publication:
Historique:
received: 29 05 2019
revised: 11 09 2019
accepted: 06 10 2019
pubmed: 7 1 2020
medline: 5 6 2020
entrez: 6 1 2020
Statut: ppublish

Résumé

In patients with hepatitis C virus (HCV) infection, the activation of the immune system by the virus or viral proteins leads to the production of numerous autoantibodies and clinical manifestations. The objectives of this study were to investigate the relationship between HCV and anti-HLA antibodies, as well as the effect of viremia on the antibody response and of direct-acting antivirals (DAAs) on anti-HLA antibody persistence in patients on the waiting list for a cadaveric kidney transplant. A total of 395 patients from the cadaveric renal transplant waiting list were included in the study. The patients were grouped according to the presence of HCV infection, and patients with HCV positivity were further divided into a spontaneous clearance group and a persistent group. Anti-HLA antibodies were examined before and after treatment of the patients in the persistent group. The One Lambda Luminex method (Thermo Fisher Scientific, Waltham, MA, United States) was used to assess both HLA class I and II alleles and the anti-HLA antibody profile. Anti-HLA class I and II antibodies were detected in 48.2% and 55.1%, respectively, of the patients infected with HCV and in 21.8% and 20.4%, respectively, of the patients who were not infected. The level of anti-HLA A3, A11, B72, B52, Cw6, Cw16, DR3, and DQ4 antibodies was significantly higher in the patients infected with HCV. There was no statistically significant difference in class I and II antibody titration between the HCV-infected spontaneous clearance group and the persistent group (class I mean fluorescence intensity [MFI] ± SD: 13,583 ± 6224, 13,450 ± 9540, P = .808; Class II MFI ± SD: 13,000 ± 8673, 8440 ± 8302, P = .317, respectively). There was no significant difference in the class I and class II anti-HLA antibody profile and titration in the persistent group after treatment with DAAs (P > .05). The results of this study demonstrated that hepatitis C DAA treatment did not change the anti-HLA antibody profile and titration.

Sections du résumé

BACKGROUND BACKGROUND
In patients with hepatitis C virus (HCV) infection, the activation of the immune system by the virus or viral proteins leads to the production of numerous autoantibodies and clinical manifestations. The objectives of this study were to investigate the relationship between HCV and anti-HLA antibodies, as well as the effect of viremia on the antibody response and of direct-acting antivirals (DAAs) on anti-HLA antibody persistence in patients on the waiting list for a cadaveric kidney transplant.
METHODS METHODS
A total of 395 patients from the cadaveric renal transplant waiting list were included in the study. The patients were grouped according to the presence of HCV infection, and patients with HCV positivity were further divided into a spontaneous clearance group and a persistent group. Anti-HLA antibodies were examined before and after treatment of the patients in the persistent group. The One Lambda Luminex method (Thermo Fisher Scientific, Waltham, MA, United States) was used to assess both HLA class I and II alleles and the anti-HLA antibody profile.
RESULTS RESULTS
Anti-HLA class I and II antibodies were detected in 48.2% and 55.1%, respectively, of the patients infected with HCV and in 21.8% and 20.4%, respectively, of the patients who were not infected. The level of anti-HLA A3, A11, B72, B52, Cw6, Cw16, DR3, and DQ4 antibodies was significantly higher in the patients infected with HCV. There was no statistically significant difference in class I and II antibody titration between the HCV-infected spontaneous clearance group and the persistent group (class I mean fluorescence intensity [MFI] ± SD: 13,583 ± 6224, 13,450 ± 9540, P = .808; Class II MFI ± SD: 13,000 ± 8673, 8440 ± 8302, P = .317, respectively). There was no significant difference in the class I and class II anti-HLA antibody profile and titration in the persistent group after treatment with DAAs (P > .05).
CONCLUSIONS CONCLUSIONS
The results of this study demonstrated that hepatitis C DAA treatment did not change the anti-HLA antibody profile and titration.

Identifiants

pubmed: 31901328
pii: S0041-1345(19)30884-X
doi: 10.1016/j.transproceed.2019.10.016
pii:
doi:

Substances chimiques

Antiviral Agents 0
Autoantibodies 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

97-101

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Adem Bayraktar (A)

Department of General Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. Electronic address: adem.bayraktar@istanbul.edu.tr.

Sebahat Usta Akgül (SU)

Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Hüseyin Bakkaloğlu (H)

Department of General Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Sonay Temurhan (S)

Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Çiğdem Kekik Çınar (ÇK)

Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Hayriye Şentürk Çiftçi (HŞ)

Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Ali Fuat Kaan Gök (AF)

Department of General Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Erol Demir (E)

Division of Nephrology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University Istanbul, Turkey.

Fatma Savran Oğuz (FS)

Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Aydın Türkmen (A)

Division of Nephrology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University Istanbul, Turkey.

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