Engineering guide RNA to reduce the off-target effects of CRISPR.

CRISPR-Cas Systems / genetics Clustered Regularly Interspaced Short Palindromic Repeats / genetics Gene Editing RNA, Guide, Kinetoplastida / genetics
CRISPR Engineered guide RNA Genome editing Off-target effects

Journal

Journal of genetics and genomics = Yi chuan xue bao
ISSN: 1673-8527
Titre abrégé: J Genet Genomics
Pays: China
ID NLM: 101304616

Informations de publication

Date de publication:
20 11 2019
Historique:
received: 18 08 2019
revised: 05 11 2019
accepted: 15 11 2019
pubmed: 7 1 2020
medline: 21 7 2020
entrez: 7 1 2020
Statut: ppublish

Résumé

As versatile and robust genome editing tools, clustered regularly interspaced short palindromic repeats (CRISPR) technologies have been broadly used in basic research, biotechnology, and therapeutic development. Off-target mutagenesis by CRISPR systems has been demonstrated, and various methods have been developed to markedly increase their specificity. In this review, we highlight the efforts of producing and modifying guide RNA (gRNA) to minimize off-target activities, including sequence and structure design, tuning expression and chemical modification. The modalities of gRNA engineering can be applied across CRISPR systems. In conjunction with CRISPR protein effectors, the engineered gRNA enables efficient and precise genome editing.

Identifiants

DOI: 10.1016/j.jgg.2019.11.003 PMID: 31902584
pubmed: 31902584
pii: S1673-8527(19)30176-6
doi: 10.1016/j.jgg.2019.11.003
pii:
doi:

Substances chimiques

RNA, Guide 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

523-529

Informations de copyright

Copyright © 2019 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Ltd. All rights reserved.

Auteurs

Jing Wu (J)

Department of Pathology, Medical Research Institute, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China; Frontier Science Center for Immunology and Metabolism, Wuhan University, 430071, China.

Hao Yin (H)

Department of Pathology, Medical Research Institute, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China; Frontier Science Center for Immunology and Metabolism, Wuhan University, 430071, China; Department of Urology, Medical Research Institute, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China. Electronic address: haoyin@whu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Engineering guide RNA to reduce the off-target...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › Engineering guide RNA to reduce the off-target...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Engineering guide RNA to reduce the off-target...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › ARN guide › Engineering guide RNA to reduce the off-target...