Sequencing and characterization of the macronuclear rDNA minichromosome of the protozoan Tetrahymena pyriformis.

Animals Chromosomes / genetics DNA Replication / genetics DNA, Ribosomal / genetics Sequence Analysis, DNA Tetrahymena pyriformis / genetics Tetrahymena thermophila / genetics
Palindrome Tetrahymena pyriformis rDNA minichromosome

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Mar 2020
Historique:
received: 12 10 2019
revised: 06 12 2019
accepted: 06 01 2020
pubmed: 14 1 2020
medline: 26 11 2020
entrez: 14 1 2020
Statut: ppublish

Résumé

Tetrahymena ribosomal DNA (rDNA) is an ideal system for studying eukaryotic DNA replication and gene transcription. In this study, we developed a new method to isolate rDNA from Tetrahymena cells and used it to sequence and annotate the complete 19,670 bp macronuclear rDNA minichromosome of Tetrahymena pyriformis, a species that lacks the germ-line micronucleus and is unable to undergo sexual reproduction. The key features of T. pyriformis and Tetrahymena thermophila rDNA sequences were then compared. Our results showed (i) the short inverted repeats (M repeats) essential for formation of rDNA minichromosome palindromic structure during sexual reproduction in Tetrahymena are highly conserved in T. pyriformis; (ii) in contrast to T. thermophila, which has two tandem domains that coordinately regulate rDNA replication, T. pyriformis has only a single domain; (iii) the 35S pre-rRNA precursor has 80.25% similarity between the two species; and (iv) the G + C content is higher in the transcribed region than the non-transcribed region in both species, but the GC-skew is more stable in T. pyriformis. The new isolation method and annotated information for the T. pyriformis rDNA minichromosome will provide a useful resource for studying DNA replication and chromosome copy number control in Tetrahymena.

Identifiants

DOI: 10.1016/j.ijbiomac.2020.01.063 PMID: 31931068
pubmed: 31931068
pii: S0141-8130(19)38265-0
doi: 10.1016/j.ijbiomac.2020.01.063
pii:
doi:

Substances chimiques

DNA, Ribosomal 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

576-581

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no conflict of interest.

Auteurs

Xiaojie Han (X)

College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Guanxiong Yan (G)

Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Yang Ma (Y)

Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Wei Miao (W)

College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; CAS Center for Excellence in Animal Evolution and Genetics, Kunming 650223, China.

Guangying Wang (G)

Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: wangguangying@ihb.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Sequencing and characterization of the...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Chromosomes › Sequencing and characterization of the...
questionsmedicales.fr › Phénomènes génétiques › Réplication de l'ADN › Sequencing and characterization of the...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN ribosomique › Sequencing and characterization of the...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Sequencing and characterization of the...
questionsmedicales.fr › Eucaryotes › Alveolata › Ciliophora › Oligohymenophorea › Hymenostomatida › Tetrahymenina › Tetrahymena › Tetrahymena pyriformis › Sequencing and characterization of the...
questionsmedicales.fr › Eucaryotes › Alveolata › Ciliophora › Oligohymenophorea › Hymenostomatida › Tetrahymenina › Tetrahymena › Tetrahymena thermophila › Sequencing and characterization of the...