Cell-to-Cell Variation in Defective Virus Expression and Effects on Host Responses during Influenza Virus Infection.
A549 Cells
Bronchi
/ cytology
Cells, Cultured
Defective Viruses
/ genetics
Epithelial Cells
/ virology
Gene Expression Profiling
Genome, Viral
Host Microbial Interactions
/ immunology
Humans
Influenza A virus
/ physiology
RNA, Viral
/ genetics
Sequence Analysis, RNA
Single-Cell Analysis
Virus Replication
defective viral genome
host immune response
influenza A virus
single-cell RNA-seq
viral transcription
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
14 01 2020
14 01 2020
Historique:
entrez:
16
1
2020
pubmed:
16
1
2020
medline:
18
9
2020
Statut:
epublish
Résumé
Virus and host factors contribute to cell-to-cell variation in viral infections and determine the outcome of the overall infection. However, the extent of the variability at the single-cell level and how it impacts virus-host interactions at a system level are not well understood. To characterize the dynamics of viral transcription and host responses, we used single-cell RNA sequencing to quantify at multiple time points the host and viral transcriptomes of human A549 cells and primary bronchial epithelial cells infected with influenza A virus. We observed substantial variability in viral transcription between cells, including the accumulation of defective viral genomes (DVGs) that impact viral replication. We show (i) a correlation between DVGs and virus-induced variation of the host transcriptional program and (ii) an association between differential inductions of innate immune response genes and attenuated viral transcription in subpopulations of cells. These observations at the single-cell level improve our understanding of the complex virus-host interplay during influenza virus infection.
Identifiants
pubmed: 31937643
pii: mBio.02880-19
doi: 10.1128/mBio.02880-19
pmc: PMC6960286
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : S10 OD018522
Pays : United States
Organisme : NIH HHS
ID : S10 OD026880
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI111598
Pays : United States
Informations de copyright
Copyright © 2020 Wang et al.
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