The First Report and Molecular Analysis of Enterocytozoon bieneusi from Raccoon (Procyon lotor) in North of Iran.
Genotyping
microsporidia
network analysis
wildlife
Journal
The Journal of eukaryotic microbiology
ISSN: 1550-7408
Titre abrégé: J Eukaryot Microbiol
Pays: United States
ID NLM: 9306405
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
14
08
2019
revised:
21
12
2019
accepted:
06
01
2020
pubmed:
17
1
2020
medline:
16
6
2021
entrez:
17
1
2020
Statut:
ppublish
Résumé
Microsporidia are known opportunistic microorganisms and usually transmitted via the fecal-oral route. However, there is no information about human-infecting microsporidia in wildlife in Iran. This study aimed to investigate and analyze human-infecting microsporidia isolated from raccoons in north of Iran. Totally, 30 fecal samples were collected; then, DNA extraction was performed and specific fragments of the SSU rRNA gene of Enterocytozoon bieneusi and Encephalitozoon species were amplified. After amplification and sequencing the ITS, the results were compared to the GenBank database. Phylogenetic trees and network analysis were employed to explore probable relationships. E. bieneusi was the only detected microsporidia among samples. Genotyping showed the genotypes D, E, and RA in 15/18 (83.33%), 1/18 (5.55%), and 2/18 (11.11%) of samples, respectively. Novel genotypes RA1 and RA2 grouped together and apart from other genotypes. E. bieneusi genotypes D and E clustered with the genotypes previously reported from animals, humans, and environmental samples. Network analysis revealed six distinct sequence types among raccoon's isolates. This study demonstrated that E. bieneusi genotype D was the most prevalent microsporidia among raccoons. It seems that wildlife may play a role in dispersion of microsporidia spores.
Substances chimiques
DNA, Fungal
0
DNA, Ribosomal
0
Banques de données
GENBANK
['MN190612', 'MN190613', 'MN190614', 'MN190615', 'MN190616', 'MN190617', 'MN190618', 'MN190619', 'MN190620', 'MN190621', 'MN190622', 'MN190623', 'MN190624', 'MN190625', 'MN190626', 'MN190627', 'MN190628', 'MN190629', 'KU852472']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
359-368Informations de copyright
© 2020 International Society of Protistologists.
Références
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