Sensitivity boosts by the CPMAS CryoProbe for challenging biological assemblies.
Capsid Proteins
/ chemistry
Carbon
/ chemistry
Cold Temperature
Escherichia coli
/ chemistry
Fungi
/ chemistry
HIV-1
/ chemistry
Humans
Hydrogen Cyanide
/ chemistry
Indicators and Reagents
Kinesins
/ chemistry
Microscopy, Electron, Transmission
Microtubules
/ chemistry
Nuclear Magnetic Resonance, Biomolecular
/ methods
Prion Proteins
/ chemistry
Sensitivity and Specificity
Temperature
Biological assemblies
CryoProbe
MAS NMR
Magic angle spinning
Journal
Journal of magnetic resonance (San Diego, Calif. : 1997)
ISSN: 1096-0856
Titre abrégé: J Magn Reson
Pays: United States
ID NLM: 9707935
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
25
09
2019
revised:
19
12
2019
accepted:
21
12
2019
pubmed:
18
1
2020
medline:
10
7
2021
entrez:
18
1
2020
Statut:
ppublish
Résumé
Despite breakthroughs in MAS NMR hardware and experimental methodologies, sensitivity remains a major challenge for large and complex biological systems. Here, we report that 3-4 fold higher sensitivities can be obtained in heteronuclear-detected experiments, using a novel HCN CPMAS probe, where the sample coil and the electronics operate at cryogenic temperatures, while the sample is maintained at ambient temperatures (BioSolids CryoProbe™). Such intensity enhancements permit recording 2D and 3D experiments that are otherwise time-prohibitive, such as 2D
Identifiants
pubmed: 31951864
pii: S1090-7807(19)30319-2
doi: 10.1016/j.jmr.2019.106680
pmc: PMC7060763
mid: NIHMS1550443
pii:
doi:
Substances chimiques
Capsid Proteins
0
Indicators and Reagents
0
KIF5B protein, human
0
Prion Proteins
0
Hydrogen Cyanide
2WTB3V159F
Carbon
7440-44-0
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
106680Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM110758
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM123743
Pays : United States
Organisme : NIAID NIH HHS
ID : P50 AI150481
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG061797
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM094357
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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