Type I IFN system activation in newborns exposed to Ro/SSA and La/SSB autoantibodies in utero.
Adult
Antibodies, Antinuclear
/ immunology
Autoantibodies
/ blood
Case-Control Studies
Echocardiography, Doppler
Female
Heart Block
/ blood
Humans
Infant, Newborn
Interferon Type I
/ blood
Male
Maternal-Fetal Exchange
/ immunology
Pregnancy
Pregnancy Complications
/ immunology
Rheumatic Diseases
/ immunology
Sweden
Transcriptome
Young Adult
Sjøgren's syndrome
autoantibodies
autoimmune diseases
Journal
RMD open
ISSN: 2056-5933
Titre abrégé: RMD Open
Pays: England
ID NLM: 101662038
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
25
04
2019
revised:
08
11
2019
accepted:
17
11
2019
entrez:
21
1
2020
pubmed:
21
1
2020
medline:
20
4
2021
Statut:
ppublish
Résumé
In utero exposure of the fetus to Ro/La autoantibodies may lead to congenital heart block (CHB). In the mother, these autoantibodies are associated with activation of the type I interferon (IFN)-system. As maternal autoantibodies are transferred to the fetus during pregnancy, we investigated whether the type I IFN-system is activated also in newborns of anti-Ro/La positive mothers, and whether fetal IFN activation is affected by maternal immunomodulatory treatment. Blood drawn at birth from anti-Ro/La positive mothers, their newborns and healthy control pairs was separated into plasma and peripheral blood mononuclear cells (PBMC). PBMC were analysed directly or cultured. mRNA expression was analysed by microarrays, cell surface markers by flow cytometry, and IFNα levels by immunoassays. We observed increased expression of IFN-regulated genes and elevated plasma IFNα levels not only in anti-Ro/La positive women, but also in their newborns. CD14 Ro/La autoantibody-exposed neonates at risk of CHB have signs of an activated immune system with an IFN signature. This study further demonstrates that neonatal cells can produce IFNα when exposed to autoantibody-containing plasma, and that maternal immunomodulatory treatment may diminish the expression of IFN-regulated genes in the fetus.
Identifiants
pubmed: 31958275
pii: rmdopen-2019-000989
doi: 10.1136/rmdopen-2019-000989
pmc: PMC7046945
pii:
doi:
Substances chimiques
Antibodies, Antinuclear
0
Autoantibodies
0
Interferon Type I
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
Déclaration de conflit d'intérêts
Competing interests: None declared.
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