Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications.
Biocatalysis
Combinatorial DNA assembly
Downstream processing
Enzyme cascade
Enzyme discovery
Enzyme engineering
Gene model
Host strain design
Process design
Whole-cell biotransformation
Journal
Biotechnology advances
ISSN: 1873-1899
Titre abrégé: Biotechnol Adv
Pays: England
ID NLM: 8403708
Informations de publication
Date de publication:
Historique:
received:
27
02
2019
revised:
18
10
2019
accepted:
13
01
2020
pubmed:
26
1
2020
medline:
7
5
2020
entrez:
26
1
2020
Statut:
ppublish
Résumé
Competitive sustainable production in industry demands new and better biocatalysts, optimized bioprocesses and cost-effective product recovery. Our review sheds light on the progress made for the individual steps towards these goals, starting with the discovery of new enzymes and their corresponding genes. The enzymes are subsequently engineered to improve their performance, combined in reaction cascades to expand the reaction scope and integrated in whole cells to provide an optimal environment for the bioconversion. Strain engineering using synthetic biology methods tunes the host for production, reaction design optimizes the reaction conditions and downstream processing ensures the efficient recovery of commercially viable products. Selected examples illustrate how modified enzymes can revolutionize future-oriented applications ranging from the bioproduction of bulk-, specialty- and fine chemicals, active pharmaceutical ingredients and carbohydrates, over the conversion of the greenhouse-gas CO
Identifiants
pubmed: 31981600
pii: S0734-9750(20)30017-3
doi: 10.1016/j.biotechadv.2020.107520
pii:
doi:
Substances chimiques
Enzymes
0
Organic Chemicals
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
107520Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.