Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration.
Golgi microtubules
STORM
cell migration
microtubule defects
post-Golgi cargo
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
04 03 2020
04 03 2020
Historique:
received:
30
04
2019
revised:
11
12
2019
accepted:
19
12
2019
pubmed:
28
1
2020
medline:
28
4
2021
entrez:
28
1
2020
Statut:
ppublish
Résumé
Microtubules derived from the Golgi (Golgi MTs) have been implicated to play critical roles in persistent cell migration, but the underlying mechanisms remain elusive, partially due to the lack of direct observation of Golgi MT-dependent vesicular trafficking. Here, using super-resolution stochastic optical reconstruction microscopy (STORM), we discovered that post-Golgi cargos are more enriched on Golgi MTs and also surprisingly move much faster than on non-Golgi MTs. We found that, compared to non-Golgi MTs, Golgi MTs are morphologically more polarized toward the cell leading edge with significantly fewer inter-MT intersections. In addition, Golgi MTs are more stable and contain fewer lattice repair sites than non-Golgi MTs. Our STORM/live-cell imaging demonstrates that cargos frequently pause at the sites of both MT intersections and MT defects. Furthermore, by optogenetic maneuvering of cell direction, we demonstrate that Golgi MTs are essential for persistent cell migration but not for cells to change direction. Together, our study unveils the role of Golgi MTs in serving as a group of "fast tracks" for anterograde trafficking of post-Golgi cargos.
Identifiants
pubmed: 31984633
doi: 10.15252/embr.201948385
pmc: PMC7054669
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e48385Subventions
Organisme : the National Key R&D program of China
ID : 2017YFA0505300
Pays : International
Organisme : the National Key R&D program of China
ID : 2017YFA0506500
Pays : International
Organisme : the National Science Foundation of China
ID : 21573013
Pays : International
Organisme : the National Science Foundation of China
ID : 21825401
Pays : International
Informations de copyright
© 2020 The Authors.
Références
Biophys J. 2002 Jun;82(6):2916-27
pubmed: 12023214
Science. 2015 Jun 5;348(6239):1155-60
pubmed: 25931445
Nat Cell Biol. 2014 Jun;16(6):561-73
pubmed: 24859005
Curr Biol. 2004 Nov 9;14(21):R931-2
pubmed: 15530387
Traffic. 2006 Jan;7(1):14-21
pubmed: 16445683
Cell. 2004 Aug 6;118(3):323-35
pubmed: 15294158
Cytometry A. 2004 Apr;58(2):167-76
pubmed: 15057970
Dev Cell. 2012 Jul 17;23(1):181-92
pubmed: 22727374
Cell Motil Cytoskeleton. 2009 Jul;66(7):365-70
pubmed: 19418559
Biophys J. 2011 Jun 8;100(11):2820-8
pubmed: 21641328
Nat Cell Biol. 2009 Sep;11(9):1069-80
pubmed: 19701196
J Cell Biol. 2011 May 30;193(5):917-33
pubmed: 21606206
Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):E7176-E7184
pubmed: 27803321
Nature. 2009 Sep 3;461(7260):104-8
pubmed: 19693014
Curr Biol. 2016 Dec 19;26(24):3399-3406
pubmed: 27916523
Curr Biol. 2010 Apr 27;20(8):697-702
pubmed: 20399099
Curr Biol. 2012 Sep 11;22(17):R673-5
pubmed: 22974990
EMBO J. 2009 Apr 22;28(8):1016-28
pubmed: 19242490
J Biol Chem. 1994 Sep 23;269(38):23769-75
pubmed: 7916345
Sci Rep. 2017 Mar 13;7:44290
pubmed: 28287156
Histochem Cell Biol. 2013 Sep;140(3):361-7
pubmed: 23821162
Dev Cell. 2016 Oct 10;39(1):44-60
pubmed: 27666745
J Cell Biol. 2014 Nov 10;207(3):323-34
pubmed: 25385183
Nat Methods. 2010 Sep;7(9):761-8
pubmed: 20729842
Mol Biol Cell. 2014 Mar;25(6):800-10
pubmed: 24478455
Nature. 2019 Apr;568(7753):546-550
pubmed: 30944468
Cytometry A. 2010 Jul;77(7):693-704
pubmed: 20583273
Science. 2007 Sep 21;317(5845):1749-53
pubmed: 17702910
Traffic. 2004 Jul;5(7):470-7
pubmed: 15180824
Cold Spring Harb Perspect Biol. 2009 Nov;1(5):a001321
pubmed: 20066116
Dev Cell. 2014 Feb 10;28(3):295-309
pubmed: 24486153
Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):E9280-E9289
pubmed: 29078376
Dev Cell. 2007 Jun;12(6):917-30
pubmed: 17543864
EMBO Rep. 2020 Mar 4;21(3):e48385
pubmed: 31984633
Science. 2008 Feb 8;319(5864):810-3
pubmed: 18174397
Nat Methods. 2012 Mar 11;9(5):493-8
pubmed: 22406856
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5686-9
pubmed: 8202549
Curr Opin Cell Biol. 2006 Oct;18(5):549-57
pubmed: 16904305
Curr Biol. 2004 Jan 20;14(2):88-98
pubmed: 14738729
Biophys J. 2016 May 24;110(10):2229-40
pubmed: 27224488
Nat Methods. 2008 Dec;5(12):1047-52
pubmed: 19029906
Nat Rev Mol Cell Biol. 2014 Sep;15(9):615-28
pubmed: 25118718
Curr Opin Cell Biol. 2010 Feb;22(1):4-13
pubmed: 20102789
J Cell Sci. 2003 Nov 15;116(Pt 22):4513-9
pubmed: 14576345
Front Neurosci. 2015 Nov 10;9:431
pubmed: 26617483
Elife. 2018 Mar 16;7:
pubmed: 29547120
J Cell Biol. 2008 Sep 8;182(5):837-43
pubmed: 18762583
Mol Biol Cell. 2009 Mar;20(6):1728-36
pubmed: 19158377
Nat Commun. 2018 Apr 16;9(1):1487
pubmed: 29662074
Eur J Cell Biol. 2009 Dec;88(12):711-7
pubmed: 19726103
Annu Rev Cell Dev Biol. 2013;29:471-99
pubmed: 23875648
PLoS Biol. 2009 Oct;7(10):e1000216
pubmed: 19823565
J Cell Biol. 2005 Jan 3;168(1):141-53
pubmed: 15631994
Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):20029-34
pubmed: 23169647
Science. 2008 Feb 22;319(5866):1086-9
pubmed: 18202255
Biophys J. 2008 Apr 15;94(8):3115-25
pubmed: 18227130
Nat Cell Biol. 2008 Apr;10(4):415-21
pubmed: 18364701
Curr Biol. 2019 Jul 22;29(14):2339-2350.e5
pubmed: 31303487
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3375-80
pubmed: 23401534
Cell Cycle. 2009 Jul 15;8(14):2168-74
pubmed: 19556895
Trends Cell Biol. 2005 Apr;15(4):183-7
pubmed: 15817373
J Biol Chem. 2007 Aug 10;282(32):23005-9
pubmed: 17576764
Cold Spring Harb Perspect Biol. 2011 May 01;3(5):
pubmed: 21504874
Exp Cell Res. 2005 Apr 15;305(1):63-73
pubmed: 15777788
Cell. 2001 Mar 23;104(6):923-35
pubmed: 11290329
Nat Cell Biol. 2014 Apr;16(4):335-44
pubmed: 24633327
Dev Cell. 2017 Nov 20;43(4):463-479.e5
pubmed: 29103954
Nat Cell Biol. 2016 Jan;18(1):54-64
pubmed: 26641717
Mol Biol Cell. 2017 Jan 15;28(2):333-345
pubmed: 27852898