Determination of Mutational Spectra Induced by Environmental Toxicants in Complex Human Cell Populations.
Benzo(a)pyrene
/ toxicity
Benzopyrenes
/ toxicity
Cell Line
Cytochrome P-450 Enzyme Inducers
/ metabolism
Denaturing Gradient Gel Electrophoresis
Electrophoresis, Polyacrylamide Gel
Exons
Guanosine
/ analogs & derivatives
Humans
Hypoxanthine Phosphoribosyltransferase
/ genetics
Mutagenesis
Mutagens
/ toxicity
Mutation
Polymerase Chain Reaction
Thionucleosides
/ pharmacology
Workflow
Benzo(a)pyrene
DGGE
Dibenzo(a,l)pyrene
HPRT
Hotspot
Human cells
Mutations
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
29
1
2020
pubmed:
29
1
2020
medline:
24
10
2020
Statut:
ppublish
Résumé
Polycyclic aromatic hydrocarbons (PAHs) are widely distributed in the environment and have potent mutagenic and carcinogenic activities. Studies of mutations induced by these compounds in human cells can help acquire an understanding of their mutagenic pathways. In this chapter, independent cultures of a human cell line expressing cytochrome P450 CYP1A1 (cell line MCL-5) were treated with benzo(a)pyrene (BaP) or dibenzo(a,l)pyrene (DBP), and mutants at the hypoxanthine phosphoribosyltransferase (HPRT) locus were selected en masse by 6-thioguanine resistance (6TG
Identifiants
pubmed: 31989563
doi: 10.1007/978-1-0716-0223-2_17
doi:
Substances chimiques
Benzopyrenes
0
Cytochrome P-450 Enzyme Inducers
0
Mutagens
0
Thionucleosides
0
Guanosine
12133JR80S
Benzo(a)pyrene
3417WMA06D
6-thioguanosine
C558LI0K8P
Hypoxanthine Phosphoribosyltransferase
EC 2.4.2.8
dibenzo(a,l)pyrene
G3X629VE4A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM