Parallel Notched Gas-Phase Enrichment for Improved Proteome Identification and Quantification with Fast Spectral Acquisition Rates.
dynamic range
gas-phase fractionation
injection waveform
label-free quantification
multinotch
orbitrap
reductive dimethylation
Journal
Journal of proteome research
ISSN: 1535-3907
Titre abrégé: J Proteome Res
Pays: United States
ID NLM: 101128775
Informations de publication
Date de publication:
02 07 2020
02 07 2020
Historique:
pubmed:
29
1
2020
medline:
22
6
2021
entrez:
29
1
2020
Statut:
ppublish
Résumé
Gas-phase fractionation enables better quantitative accuracy, improves signal-to-noise ratios, and increases sensitivity in proteomic analyses. However, traditional gas-phase enrichment, which relies upon a large continuous bin, results in suboptimal enrichment, as most chromatographic separations are not 100% orthogonal relative to the first MS dimension (MS
Identifiants
pubmed: 31990573
doi: 10.1021/acs.jproteome.9b00715
pmc: PMC7334078
mid: NIHMS1595924
doi:
Substances chimiques
Ions
0
Peptides
0
Proteome
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2750-2757Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM067945
Pays : United States
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