Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns.
Chromatography, Affinity
/ methods
Chromatography, High Pressure Liquid
Histidine
/ chemistry
Humans
Microtubule-Associated Proteins
/ chemistry
Nitrilotriacetic Acid
/ chemistry
Oligopeptides
/ chemistry
Peptides
/ analysis
Protein Binding
Protein Interaction Maps
/ physiology
Proteomics
/ methods
Recombinant Fusion Proteins
/ biosynthesis
Tandem Mass Spectrometry
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
03 03 2020
03 03 2020
Historique:
pubmed:
30
1
2020
medline:
14
1
2021
entrez:
30
1
2020
Statut:
ppublish
Résumé
We describe an affinity purification-mass spectrometry (AP-MS) method for probing the interactome of a special targeting protein. The AP was implemented with monolithic micro immobilized metal ion affinity chromatography columns (m-IMAC) which were prepared by photoinitiated polymerization in the tip of a pipet (spin-tip columns). The recombinant His
Identifiants
pubmed: 31992042
doi: 10.1021/acs.analchem.9b05355
doi:
Substances chimiques
FYCO1 protein, human
0
His-His-His-His-His-His
0
Microtubule-Associated Proteins
0
Oligopeptides
0
Peptides
0
Recombinant Fusion Proteins
0
Histidine
4QD397987E
Nitrilotriacetic Acid
KA90006V9D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM