Pre-emptive genomic surveillance of emerging ebolaviruses.
Ebolavirus
PCR
genomic surveillance
nested PCR
Journal
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
ISSN: 1560-7917
Titre abrégé: Euro Surveill
Pays: Sweden
ID NLM: 100887452
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
entrez:
30
1
2020
pubmed:
30
1
2020
medline:
20
2
2020
Statut:
ppublish
Résumé
Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. Our approach enables pre-emptive genomic surveillance of ongoing and future ebolavirus outbreaks irrespective of variant divergence.
Identifiants
pubmed: 31992392
doi: 10.2807/1560-7917.ES.2020.25.3.1900765
pmc: PMC6988270
doi:
Substances chimiques
DNA, Viral
0
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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