Human proteinase 3 resistance to inhibition extends to alpha-2 macroglobulin.
Amino Acid Sequence
Binding Sites
Chromatography, Liquid
/ methods
Humans
Kinetics
Mass Spectrometry
/ methods
Molecular Docking Simulation
Myeloblastin
/ chemistry
Peptides
/ chemistry
Pregnancy-Associated alpha 2-Macroglobulins
/ chemistry
Protein Binding
Protein Domains
Proteolysis
Recombinant Proteins
/ chemistry
proteinase 3
proteolysis
serine proteinase
α2-macroglobulin
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
27
10
2019
revised:
12
12
2019
accepted:
27
01
2020
pubmed:
30
1
2020
medline:
18
5
2021
entrez:
30
1
2020
Statut:
ppublish
Résumé
Polymorphonuclear neutrophils contain at least four serine endopeptidases, namely neutrophil elastase (NE), proteinase 3 (PR3), cathepsin G (CatG), and NSP4, which contribute to the regulation of infection and of inflammatory processes. In physiological conditions, endogenous inhibitors including α2-macroglobulin (α2-M), serpins [α1-proteinase inhibitor (α1-PI)], monocyte neutrophil elastase inhibitor (MNEI), α1-antichymotrypsin, and locally produced chelonianins (elafin, SLPI) control excessive proteolytic activity of neutrophilic serine proteinases. In contrast to human NE (hNE), hPR3 is weakly inhibited by α1-PI and MNEI but not by SLPI. α2-M is a large spectrum inhibitor that traps a variety of proteinases in response to cleavage(s) in its bait region. We report here that α2-M was more rapidly processed by hNE than hPR3 or hCatG. This was confirmed by the observation that the association between α2-M and hPR3 is governed by a k
Substances chimiques
Peptides
0
Pregnancy-Associated alpha 2-Macroglobulins
0
Recombinant Proteins
0
Myeloblastin
EC 3.4.21.76
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4068-4081Informations de copyright
© 2020 INSERM UMR. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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