Synergistic defects in pre-rRNA processing from mutations in the U3-specific protein Rrp9 and U3 snoRNA.
Mutation
Nuclear Proteins
/ metabolism
Protein Interaction Domains and Motifs
Protein Interaction Mapping
RNA Precursors
/ metabolism
RNA Processing, Post-Transcriptional
RNA, Ribosomal, 18S
/ metabolism
RNA, Small Nucleolar
/ chemistry
RNA-Binding Proteins
/ metabolism
Ribonucleoproteins, Small Nucleolar
/ chemistry
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ metabolism
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
17 04 2020
17 04 2020
Historique:
accepted:
22
01
2020
revised:
17
01
2020
received:
19
07
2019
pubmed:
31
1
2020
medline:
7
7
2020
entrez:
31
1
2020
Statut:
ppublish
Résumé
U3 snoRNA and the associated Rrp9/U3-55K protein are essential for 18S rRNA production by the SSU-processome complex. U3 and Rrp9 are required for early pre-rRNA cleavages at sites A0, A1 and A2, but the mechanism remains unclear. Substitution of Arg 289 in Rrp9 to Ala (R289A) specifically reduced cleavage at sites A1 and A2. Surprisingly, R289 is located on the surface of the Rrp9 β-propeller structure opposite to U3 snoRNA. To understand this, we first characterized the protein-protein interaction network of Rrp9 within the SSU-processome. This identified a direct interaction between the Rrp9 β-propeller domain and Rrp36, the strength of which was reduced by the R289A substitution, implicating this interaction in the observed processing phenotype. The Rrp9 R289A mutation also showed strong synergistic negative interactions with mutations in U3 that destabilize the U3/pre-rRNA base-pair interactions or reduce the length of their linking segments. We propose that the Rrp9 β-propeller and U3/pre-rRNA binding cooperate in the structure or stability of the SSU-processome. Additionally, our analysis of U3 variants gave insights into the function of individual segments of the 5'-terminal 72-nt sequence of U3. We interpret these data in the light of recently reported SSU-processome structures.
Identifiants
pubmed: 31996908
pii: 5717751
doi: 10.1093/nar/gkaa066
pmc: PMC7144924
doi:
Substances chimiques
Nuclear Proteins
0
RNA Precursors
0
RNA, Ribosomal, 18S
0
RNA, Small Nucleolar
0
RNA, U3 small nucleolar
0
RNA-Binding Proteins
0
RRP36 protein, S cerevisiae
0
RRP5 protein, S cerevisiae
0
Ribonucleoproteins, Small Nucleolar
0
SGD1 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
ribonucleoprotein, U3 small nucleolar
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3848-3868Subventions
Organisme : Wellcome Trust
ID : 077248
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203149
Pays : United Kingdom
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
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