Development of a new chromogenic method for putrescine quantification using coupling reactions involving putrescine oxidase.

Ampyrone / chemistry Chromogenic Compounds / chemistry Colorimetry / methods Oxidoreductases Acting on CH-NH Group Donors / chemistry Proteus mirabilis / metabolism Putrescine / analysis Rhodococcus / enzymology

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
15 03 2020
Historique:
received: 15 03 2019
revised: 13 01 2020
accepted: 27 01 2020
pubmed: 2 2 2020
medline: 13 1 2021
entrez: 2 2 2020
Statut: ppublish

Résumé

Quantification of polyamines, including putrescine, is generally performed using high-performance liquid chromatography (HPLC) or gas chromatography. However, these methods are time-consuming because of sample derivatization and analytical reagent preparation. In this study, we developed a simple and high-throughput putrescine quantification method on a 96-well microtiter plate using putrescine oxidase from Rhodococcus erythropolis NCIMB 11540, peroxidase, 4-aminoantipyrine, and N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt. The developed method (named as PuO-POD-4AA-TOPS method) was applicable to bacterial culture supernatants. Furthermore, putrescine concentrations determined by the developed method roughly corresponded to the concentrations determined by HPLC.

Identifiants

DOI: 10.1016/j.ab.2020.113607 PMID: 32006471
pubmed: 32006471
pii: S0003-2697(19)30262-3
doi: 10.1016/j.ab.2020.113607
pii:
doi:

Substances chimiques

Chromogenic Compounds 0
Ampyrone 0M0B7474RA
putrescine oxidase EC 1.4.3.10
Oxidoreductases Acting on CH-NH Group Donors EC 1.5.-
Putrescine V10TVZ52E4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

113607

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Yuta Sugiyama (Y)

Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, 921-8836, Ishikawa, Japan. Electronic address: yusugi@ishikawa-pu.ac.jp.

Hirokazu Ohta (H)

Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, 921-8836, Ishikawa, Japan. Electronic address: p1794001@ishikawa-pu.ac.jp.

Rika Hirano (R)

Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, 921-8836, Ishikawa, Japan. Electronic address: rikahirano@outlook.com.

Hiromi Shimokawa (H)

Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, 921-8836, Ishikawa, Japan. Electronic address: shim0_000@yahoo.co.jp.

Mikiyasu Sakanaka (M)

Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, 921-8836, Ishikawa, Japan. Electronic address: sakanaka@ishikawa-pu.ac.jp.

Takashi Koyanagi (T)

Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, 921-8836, Ishikawa, Japan. Electronic address: koyataka@ishikawa-pu.ac.jp.

Shin Kurihara (S)

Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, 921-8836, Ishikawa, Japan. Electronic address: skurihara@waka.kindai.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Azoles › Pyrazoles › Pyrazolones › Aminophénazone › 4-Amino-1,5-diméthyl-2-phényl-1,2-dihydro-3H-pyrazol-3-one › Development of a new chromogenic method for...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Réactifs chromogènes › Development of a new chromogenic method for...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Titrimétrie › Colorimétrie › Development of a new chromogenic method for...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxidoreductases acting on CH-NH group donors › Development of a new chromogenic method for...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Proteus › Proteus mirabilis › Development of a new chromogenic method for...
questionsmedicales.fr › Composés chimiques organiques › Amines › Polyamines › Diamines › Putrescine › Development of a new chromogenic method for...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Nocardiaceae › Rhodococcus › Development of a new chromogenic method for...