Addition of fish oil to atherogenic high fat diet inhibited atherogenesis while olive oil did not, in LDL receptor KO mice.
Animals
Atherosclerosis
/ blood
Biomarkers
/ blood
Chemokine CCL2
/ metabolism
Cholesterol
/ blood
Diet, High-Fat
Disease Models, Animal
Docosahexaenoic Acids
/ administration & dosage
Eicosapentaenoic Acid
/ administration & dosage
Female
Interleukin-6
/ metabolism
Liver
/ metabolism
Mice, Inbred C57BL
Mice, Knockout
Non-alcoholic Fatty Liver Disease
/ blood
Oleic Acid
/ administration & dosage
Olive Oil
/ administration & dosage
Plaque, Atherosclerotic
Receptors, LDL
/ deficiency
Time Factors
Atherosclerotic lesion
Fish oil
High fat diet
LDL receptor knockout mice
Nonalcoholic fatty liver disease
Olive oil
Journal
Nutrition, metabolism, and cardiovascular diseases : NMCD
ISSN: 1590-3729
Titre abrégé: Nutr Metab Cardiovasc Dis
Pays: Netherlands
ID NLM: 9111474
Informations de publication
Date de publication:
12 04 2020
12 04 2020
Historique:
received:
27
03
2019
revised:
18
09
2019
accepted:
06
12
2019
pubmed:
3
2
2020
medline:
15
9
2020
entrez:
3
2
2020
Statut:
ppublish
Résumé
Mediterranean diet has been associated with decreased cardiovascular morbidity and mortality. Both fish and olive oil are key components of this diet. Therefore, we compared their effects on nonalcoholic fatty liver disease (NAFLD) and atherogenesis in a mouse model, fed a high fat diet. Forty nine, female LDL receptor knockout (LDLR KO) mice were allocated into 3 groups and fed an atherogenic high fat (HF) diet for 9 weeks. The HF group was fed a high fat diet alone. A HF + OO group was fed a HF diet with added olive oil (60 ml/kg feed), and the third group (HF + FO) was fed a HF diet with added fish oil (60 ml/kg feed). Both additions of fish and olive oil, significantly decreased plasma cholesterol elevation compared to HF diet. Nevertheless, only fish oil addition reduced significantly atherosclerotic lesion area by 51% compared to HF group. Liver levels of eicosapentenoic (EPA) and docosahexaenoic (DHA) acids were several folds higher in HF + FO group than in HF and HF + OO groups. Liver levels of oleic acid were higher in HF + OO compared to the other groups. Moreover, Fish oil addition significantly decreased NAFLD scores related to steatosis and inflammation and lowered the expression of the inflammatory genes interleukin 6 (IL6) and monocyte chemoattractant protein 1 (MCP1). These results suggest that fish oil addition on top of an atherogenic, HF diet, is beneficial, while olive oil is not, in its effect on plaque formation and NAFLD in LDLR KO mice.
Sections du résumé
BACKGROUND AND AIMS
Mediterranean diet has been associated with decreased cardiovascular morbidity and mortality. Both fish and olive oil are key components of this diet. Therefore, we compared their effects on nonalcoholic fatty liver disease (NAFLD) and atherogenesis in a mouse model, fed a high fat diet.
METHODS AND RESULTS
Forty nine, female LDL receptor knockout (LDLR KO) mice were allocated into 3 groups and fed an atherogenic high fat (HF) diet for 9 weeks. The HF group was fed a high fat diet alone. A HF + OO group was fed a HF diet with added olive oil (60 ml/kg feed), and the third group (HF + FO) was fed a HF diet with added fish oil (60 ml/kg feed). Both additions of fish and olive oil, significantly decreased plasma cholesterol elevation compared to HF diet. Nevertheless, only fish oil addition reduced significantly atherosclerotic lesion area by 51% compared to HF group. Liver levels of eicosapentenoic (EPA) and docosahexaenoic (DHA) acids were several folds higher in HF + FO group than in HF and HF + OO groups. Liver levels of oleic acid were higher in HF + OO compared to the other groups. Moreover, Fish oil addition significantly decreased NAFLD scores related to steatosis and inflammation and lowered the expression of the inflammatory genes interleukin 6 (IL6) and monocyte chemoattractant protein 1 (MCP1).
CONCLUSION
These results suggest that fish oil addition on top of an atherogenic, HF diet, is beneficial, while olive oil is not, in its effect on plaque formation and NAFLD in LDLR KO mice.
Identifiants
pubmed: 32007335
pii: S0939-4753(19)30454-5
doi: 10.1016/j.numecd.2019.12.007
pii:
doi:
Substances chimiques
Biomarkers
0
Ccl2 protein, mouse
0
Chemokine CCL2
0
Interleukin-6
0
Olive Oil
0
Receptors, LDL
0
interleukin-6, mouse
0
Docosahexaenoic Acids
25167-62-8
Oleic Acid
2UMI9U37CP
Cholesterol
97C5T2UQ7J
Eicosapentaenoic Acid
AAN7QOV9EA
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
709-716Informations de copyright
Copyright © 2019 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no conflict of interest.