The distribution of oxytocin and the oxytocin receptor in rat brain: relation to regions active in migraine.
CGRP, CGRP receptors
Immunohistochemistry
Migraine-related regions
Oxytocin
Oxytocin receptor
Journal
The journal of headache and pain
ISSN: 1129-2377
Titre abrégé: J Headache Pain
Pays: England
ID NLM: 100940562
Informations de publication
Date de publication:
07 Feb 2020
07 Feb 2020
Historique:
received:
01
10
2019
accepted:
23
01
2020
entrez:
8
2
2020
pubmed:
8
2
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Recent work, both clinical and experimental, suggests that the hypothalamic hormone oxytocin (OT) and its receptor (OTR) may be involved in migraine pathophysiology. In order to better understand possible central actions of OT in migraine/headache pathogenesis, we mapped the distribution of OT and OTR in nerve cells and fibers in rat brain with a focus on areas related to migraine attacks and/or shown previously to contain calcitonin gene related peptide (CGRP), another neuropeptide involved in migraine. Distribution of OT and OTR in the adult, rat brain was qualitatively examined with immunohistochemistry using a series of well characterized specific antibodies. As expected, OT was extensively localized in the cell somas of two hypothalamic nuclei, the supraoptic (SO or SON) and paraventricular nuclei (Pa or PVN). OT also was found in many other regions of the brain where it was localized mainly in nerve fibers. In contrast, OTR staining in the brain was mainly observed in cell somas with very little expression in fibers. The most distinct OTR expression was found in the hippocampus, the pons and the substantia nigra. In some regions of the brain (e.g. the amygdala and the hypothalamus), both OT and OTR were expressed (match). Mismatch between the peptide and its receptor was primarily observed in the cerebral and cerebellar cortex (OT expression) and hippocampus (OTR expression). We compared OT/OTR distribution in the CNS with that of CGRP and identified regions related to migraine. In particular, regions suggested as "migraine generators", showed correspondence among the three mappings. These findings suggest central OT pathways may contribute to the role of the hypothalamus in migraine attacks.
Sections du résumé
BACKGROUND
BACKGROUND
Recent work, both clinical and experimental, suggests that the hypothalamic hormone oxytocin (OT) and its receptor (OTR) may be involved in migraine pathophysiology. In order to better understand possible central actions of OT in migraine/headache pathogenesis, we mapped the distribution of OT and OTR in nerve cells and fibers in rat brain with a focus on areas related to migraine attacks and/or shown previously to contain calcitonin gene related peptide (CGRP), another neuropeptide involved in migraine.
METHODS
METHODS
Distribution of OT and OTR in the adult, rat brain was qualitatively examined with immunohistochemistry using a series of well characterized specific antibodies.
RESULTS
RESULTS
As expected, OT was extensively localized in the cell somas of two hypothalamic nuclei, the supraoptic (SO or SON) and paraventricular nuclei (Pa or PVN). OT also was found in many other regions of the brain where it was localized mainly in nerve fibers. In contrast, OTR staining in the brain was mainly observed in cell somas with very little expression in fibers. The most distinct OTR expression was found in the hippocampus, the pons and the substantia nigra. In some regions of the brain (e.g. the amygdala and the hypothalamus), both OT and OTR were expressed (match). Mismatch between the peptide and its receptor was primarily observed in the cerebral and cerebellar cortex (OT expression) and hippocampus (OTR expression).
CONCLUSIONS
CONCLUSIONS
We compared OT/OTR distribution in the CNS with that of CGRP and identified regions related to migraine. In particular, regions suggested as "migraine generators", showed correspondence among the three mappings. These findings suggest central OT pathways may contribute to the role of the hypothalamus in migraine attacks.
Identifiants
pubmed: 32028899
doi: 10.1186/s10194-020-1079-8
pii: 10.1186/s10194-020-1079-8
pmc: PMC7006173
doi:
Substances chimiques
Receptors, Oxytocin
0
Oxytocin
50-56-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
10Subventions
Organisme : Hjärt-Lungfonden
ID : none
Organisme : International Headache Society
ID : none
Références
J Neuroendocrinol. 1998 Dec;10(12):881-95
pubmed: 9870745
Physiol Rev. 1993 Jul;73(3):489-527
pubmed: 8392735
Clin J Pain. 2014 May;30(5):453-62
pubmed: 23887343
Neuroscience. 2013 Dec 3;253:155-64
pubmed: 24012742
Physiol Rev. 2001 Apr;81(2):629-83
pubmed: 11274341
Lancet. 2017 Sep 16;390(10100):1211-1259
pubmed: 28919117
J Neuroendocrinol. 2017 Oct 12;:
pubmed: 29024187
Neurosci Biobehav Rev. 2020 Jan;108:1-23
pubmed: 31647964
Brain Res Brain Res Rev. 2005 Jun;48(3):438-56
pubmed: 15914251
Neuropeptides. 2013 Apr;47(2):93-7
pubmed: 23375440
Nat Rev Neurosci. 2006 Feb;7(2):126-36
pubmed: 16429122
Neurol Sci. 2017 May;38(Suppl 1):125-130
pubmed: 28527054
Front Neurosci. 2013 Mar 15;7:35
pubmed: 23508240
Cell Tissue Res. 2019 Jan;375(1):41-48
pubmed: 30498946
Headache. 2017 May;57 Suppl 2:64-75
pubmed: 28485846
Cell Tissue Res. 1975 Dec 2;164(2):153-62
pubmed: 53102
Cephalalgia. 2019 Mar;39(3):342-353
pubmed: 28856910
Annu Rev Neurosci. 1983;6:269-324
pubmed: 6132586
J Neurosci. 2013 Sep 11;33(37):14869-77
pubmed: 24027286
Nat Rev Neurol. 2016 Aug;12(8):455-64
pubmed: 27389092
Genes Brain Behav. 2019 Jan;18(1):e12535
pubmed: 30378258
J Neurosci Res. 2017 Jan 2;95(1-2):719-730
pubmed: 27870443
Cephalalgia. 2013 Jun;33(8):593-603
pubmed: 23671255
Science. 2004 Apr 2;304(5667):108-10
pubmed: 15064420
Front Neuroendocrinol. 2004 Sep-Dec;25(3-4):150-76
pubmed: 15589267
Exp Neurol. 2020 Jan;323:113079
pubmed: 31678349
Biol Psychiatry. 2016 Feb 1;79(3):155-64
pubmed: 26001309
Neuropharmacology. 2018 Feb;129:109-117
pubmed: 29169960
J Comp Neurol. 1980 Dec 1;194(3):555-70
pubmed: 7451682
Cell Tissue Res. 1978 Sep 26;192(3):423-35
pubmed: 699026
Neuroendocrinology. 1980 Dec;31(6):410-7
pubmed: 6109264
Bioessays. 1998 Sep;20(9):741-9
pubmed: 9819563
J Physiol. 1956 Aug 28;133(2):330-3
pubmed: 13358075
J Neurosci. 1989 May;9(5):1764-73
pubmed: 2542479
Front Neuroendocrinol. 2016 Jan;40:1-23
pubmed: 25951955
Neuroscience. 2003;122(1):155-67
pubmed: 14596857
Headache. 2018 Jan;58(1):62-77
pubmed: 29094347
Neuron. 2016 Mar 16;89(6):1291-1304
pubmed: 26948889
Dev Neurobiol. 2017 Feb;77(2):169-189
pubmed: 27626613
Nat Rev Neurol. 2018 Jun;14(6):338-350
pubmed: 29691490
Cell Tissue Res. 1989;256(2):411-7
pubmed: 2731224
Cephalalgia. 2016 Sep;36(10):943-50
pubmed: 26590611
Neuroscience. 1989;32(2):435-9
pubmed: 2586758