Liquid-liquid chromatography in sample pretreatment for quantitative analysis of trace component in traditional Chinese medicines by conventional liquid chromatography.
Liquid chromatography
Liquid-liquid chromatography
Quantitative determination
Sample pretreatment
Triperygium wilfordii Hook.f
Wilforlide A
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
24 May 2020
24 May 2020
Historique:
received:
28
11
2019
revised:
19
01
2020
accepted:
22
01
2020
pubmed:
11
2
2020
medline:
2
7
2020
entrez:
11
2
2020
Statut:
ppublish
Résumé
A method for sample pretreatment using liquid-liquid chromatography combined with a conventional liquid chromatography was developed for quantitative determination of trace chemical component in traditional Chinese medicine. The main effective component, wilforlide A, in the traditional Chinese medicinal herb Tripterygium wilfordii as well as in its Chinese patent medicine glycosides tablets was successfully determined after sample pretreatment by liquid-liquid chromatography. A biphasic solvent system n-hexane-ethyl acetate-ethanol-water (6:4:6:4, v/v) was screened for crude sample treatment by liquid-liquid chromatography. The collection time of eluted fractions containing target component could be well predicted using a continuous-stirred tank reactors model after determination of its retention time. Then, quantitative analysis of wilforlide A in Tripterygium wilfordii as well as in its tablets could be successfully determined by conventional reversed-phase high performance liquid chromatography with UV detector. Under the optimized conditions, the method showed good linearity (R
Identifiants
pubmed: 32037073
pii: S0021-9673(20)30094-7
doi: 10.1016/j.chroma.2020.460917
pii:
doi:
Substances chimiques
Drugs, Chinese Herbal
0
Glycosides
0
Oleanolic Acid
6SMK8R7TGJ
wilforlide A
84104-71-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
460917Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.