A novel pre-CTX prophage in the Vibrio cholerae serogroup O139 strain.
CTXΦ
Genome
Lysogenic conversion
Prophage
Vibrio cholerae
Journal
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
ISSN: 1567-7257
Titre abrégé: Infect Genet Evol
Pays: Netherlands
ID NLM: 101084138
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
08
10
2019
revised:
16
01
2020
accepted:
07
02
2020
pubmed:
12
2
2020
medline:
29
6
2021
entrez:
12
2
2020
Statut:
ppublish
Résumé
In Vibrio cholerae, the lysogenic bacteriophage CTXΦ carries the cholera toxin genes ctxAB, which can be transferred from toxigenic strains to nontoxigenic strains through infection and lysogenic conversion of CTXΦ. This phage also has the precursor genome which does not harbor ctxAB, named pre-CTXΦ. Based on the sequences of the transcriptional regulator-encoding gene rstR alleles in CTXΦ/pre-CTXΦ, multiple types of these prophages have been classified and identified in toxigenic and nontoxigenic V. cholerae strains. In this study, by combining the short-read and long-read sequencing approaches of next generation sequencing, we obtained the complete genome sequence of the studied V. cholerae toxigenic serogroup O139 strain and identified the CTXΦ and a pre-CTXΦ genome type encoding a novel rstR allele, pre-CTX
Identifiants
pubmed: 32045711
pii: S1567-1348(20)30070-8
doi: 10.1016/j.meegid.2020.104238
pii:
doi:
Substances chimiques
DNA, Viral
0
Viral Proteins
0
Cholera Toxin
9012-63-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104238Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest We have no conflict of interest to declare for this study.