Virulence properties and antimicrobial resistance of Pseudomonas aeruginosa isolated from cave waters at Roraima Tepui, Guayana Highlands.
Anti-Bacterial Agents
/ pharmacology
Biofilms
/ growth & development
Caves
/ microbiology
Drug Resistance, Bacterial
/ drug effects
Ecosystem
Microbial Sensitivity Tests
Phylogeny
Pseudomonas aeruginosa
/ drug effects
Pyocyanine
/ biosynthesis
Quorum Sensing
RNA, Ribosomal, 16S
/ genetics
Venezuela
Virulence
Virulence Factors
/ genetics
Water Microbiology
Pseudomonads
biofilms
resistance
virulence
water
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
27
11
2019
revised:
05
02
2020
accepted:
06
02
2020
pubmed:
13
2
2020
medline:
9
4
2020
entrez:
13
2
2020
Statut:
ppublish
Résumé
Pseudomonas aeruginosa is a prominent member of emerging waterborne pathogens. The environmental reservoirs of multi-resistant phenotypes and other virulence factors in this bacterium are poorly understood. Our study aimed to determine the virulence properties of P. aeruginosa isolated from Roraima Sur Cave (RSC) waters at Guayana Highlands. Based on the best identification at species level by biochemical tests, 16S rRNA sequencing and phylogenetic inferences, one RSC isolate named LG11 was characterized for virulence properties in comparison with P. aeruginosa reference strains. PCR amplification of alginate, elastase, exoenzyme S, exotoxin A, neuraminidase and Quorum-Sensing genes showed a high virulence potential in LG11. This isolate demonstrated multi-resistance to ceftriaxone, tigecycline and imipenem. Pyocyanin production was greater in LG11 (0·478 µg ml
Substances chimiques
Anti-Bacterial Agents
0
RNA, Ribosomal, 16S
0
Virulence Factors
0
Pyocyanine
9OQM399341
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
372-379Subventions
Organisme : DID-USB
Organisme : IVIC
Informations de copyright
© 2020 The Society for Applied Microbiology.
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